Comparative proteomic analysis of Listeria monocytogenes strains F2365 and EGD

Janet R. Donaldson, Bindu Nanduri, Shane C Burgess, Mark L. Lawrence

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Listeria monocytogenes is a gram-positive, food-borne pathogen that causes disease in both humans and animals. There are three major genetic lineages of L. monocytogenes and 13 serovars. To further our understanding of the differences that exist between different genetic lineages/serovars of L. monocytogenes, we analyzed the global protein expression of the serotype 1/2a strain EGD and the serotype 4b strain F2365 during early-stationary-phase growth at 37°C. Using multidimensional protein identification technology with electrospray ionization tandem mass spectrometry, we identified 1,754 proteins from EGD and 1,427 proteins from F2365, of which 1,077 were common to both. Analysis of proteins that had significantly altered expression between strains revealed potential biological differences between these two L. monocytogenes strains. In particular, the strains differed in expression of proteins involved in cell wall physiology and flagellar biosynthesis, as well as DNA repair proteins and stress response proteins.

Original languageEnglish (US)
Pages (from-to)366-373
Number of pages8
JournalApplied and Environmental Microbiology
Volume75
Issue number2
DOIs
StatePublished - Jan 2009
Externally publishedYes

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proteomics
Listeria monocytogenes
Proteomics
protein
serotypes
Proteins
proteins
protein synthesis
diseases and disorders (animals and humans)
Cell Physiological Phenomena
food pathogens
DNA repair
Electrospray Ionization Mass Spectrometry
Heat-Shock Proteins
Tandem Mass Spectrometry
analysis
stress response
DNA Repair
Cell Wall
physiology

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Comparative proteomic analysis of Listeria monocytogenes strains F2365 and EGD. / Donaldson, Janet R.; Nanduri, Bindu; Burgess, Shane C; Lawrence, Mark L.

In: Applied and Environmental Microbiology, Vol. 75, No. 2, 01.2009, p. 366-373.

Research output: Contribution to journalArticle

Donaldson, Janet R. ; Nanduri, Bindu ; Burgess, Shane C ; Lawrence, Mark L. / Comparative proteomic analysis of Listeria monocytogenes strains F2365 and EGD. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 2. pp. 366-373.
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