Comparative proteomics of mouse tears and saliva: Evidence from large protein families for functional adaptation

Robert C. Karn, Christina M Laukaitis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We produced a tear proteome of the genome mouse, C57BL/6, that contained 139 different protein identifications: 110 from a two-dimensional (2D) gel with subsequent trypsin digestion, 19 from a one-dimensional (1D) gel with subsequent trypsin digestion and ten from a 1D gel with subsequent Asp-N digestion. We compared this tear proteome with a C57BL/6 mouse saliva proteome produced previously. Sixteen of the 139 tear proteins are shared between the two proteomes, including six proteins that combat microbial growth. Among the 123 other tear proteins, were members of four large protein families that have no counterparts in humans: Androgen-binding proteins (ABPs) with different members expressed in the two proteomes, Exocrine secreted peptides (ESPs) expressed exclusively in the tear proteome, major urinary proteins (MUPs) expressed in one or both proteomes and the mouse-specific Kallikreins (subfamily b KLKs) expressed exclusively in the saliva proteome. All four families have members with suggested roles in mouse communication, which may influence some aspect of reproductive behavior. We discuss this in the context of functional adaptation involving tear and saliva proteins in the secretions of mouse lacrimal and salivary glands, respectively.

Original languageEnglish (US)
Pages (from-to)283-297
Number of pages15
JournalProteomes
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2015

Fingerprint

Proteome
Tears
Saliva
Proteomics
Proteins
Digestion
Gels
Inbred C57BL Mouse
Trypsin
Androgen-Binding Protein
Reproductive Behavior
Lacrimal Apparatus
Kallikreins
Salivary Glands
Genes
Communication
Genome
Peptides
Growth
tear proteins

Keywords

  • Adaptation
  • Lacrimal gland
  • Mouse
  • Proteome
  • Saliva
  • Salivary glands
  • Tears

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Comparative proteomics of mouse tears and saliva : Evidence from large protein families for functional adaptation. / Karn, Robert C.; Laukaitis, Christina M.

In: Proteomes, Vol. 3, No. 3, 01.09.2015, p. 283-297.

Research output: Contribution to journalArticle

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