Comparative study of antibody immobilization mediated by lipid and polymer fibers

Celine Cohn, Siu Ling Leung, Zhengbao Zha, Jessica Crosby, Weibing Teng, Xiaoyi Wu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Antibody immobilization and function retention are important to a variety of applications, including proteomics, drug discovery, diagnostics, and biosensors. The present study investigates antibody immobilization mediated by cholesteryl succinyl silane (CSS) fibers, in comparison to hydrophobic polycaprolactone (PCL) fibers and hydrophilic plasma-treated PCL fibers. When incubated with a model protein, the formation of protein aggregates is observed on hydrophobic PCL fibers but not on the more hydrophobic CSS fibers, indicating that CSS fibers immobilize proteins through mechanisms other than hydrophobic interaction. When exposed to a limited amount of antibody, CSS fibers immobilize more antibodies than plasma-treated PCL fibers and no fewer antibodies than PCL fibers. The function retention of antibodies immobilized on the fibers is analyzed using a cell-capture assay, which shows that the antibody-functionalized CSS fibrous matrices capture 6- or 7-fold more cells than the antibody-functionalized PCL or plasma-treated PCL fibrous matrices, respectively. Data collected from the study show that the lipid fiber-mediated immobilization of antibody not only maintains the advantages of physical immobilization such as easiness and rapidness of operation but also improves function retention.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume134
DOIs
StatePublished - Oct 1 2015

Fingerprint

antibodies
immobilization
Antibodies
Immobilization
Lipids
lipids
Polymers
Silanes
Polycaprolactone
fibers
Fibers
polymers
silanes
proteins
Proteins
Plasmas
Physical Restraint
Immobilized Antibodies
Biosensing Techniques
Drug Discovery

Keywords

  • Antibody immobilization
  • Cholesterol
  • Function retention
  • Lipid fibers
  • Physical adsorption

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Comparative study of antibody immobilization mediated by lipid and polymer fibers. / Cohn, Celine; Leung, Siu Ling; Zha, Zhengbao; Crosby, Jessica; Teng, Weibing; Wu, Xiaoyi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 134, 01.10.2015, p. 1-7.

Research output: Contribution to journalArticle

Cohn, Celine ; Leung, Siu Ling ; Zha, Zhengbao ; Crosby, Jessica ; Teng, Weibing ; Wu, Xiaoyi. / Comparative study of antibody immobilization mediated by lipid and polymer fibers. In: Colloids and Surfaces B: Biointerfaces. 2015 ; Vol. 134. pp. 1-7.
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