Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses

C. Pettit, L. L. Minnich, Z. M. Shehab, C. G. Ray

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Abstract

Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.

Original languageEnglish (US)
Pages (from-to)1325-1326
Number of pages2
JournalJournal of clinical microbiology
Volume25
Issue number7
StatePublished - Jan 1 1987

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ASJC Scopus subject areas

  • Microbiology (medical)

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