Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses

C. Pettit, L. L. Minnich, Ziad M Shehab, C. G. Ray

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.

Original languageEnglish (US)
Pages (from-to)1325-1326
Number of pages2
JournalJournal of Clinical Microbiology
Volume25
Issue number7
StatePublished - 1987

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Poliovirus
Indirect Fluorescent Antibody Technique
Antibodies
Serum

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses. / Pettit, C.; Minnich, L. L.; Shehab, Ziad M; Ray, C. G.

In: Journal of Clinical Microbiology, Vol. 25, No. 7, 1987, p. 1325-1326.

Research output: Contribution to journalArticle

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