Comparison of BGM and PLC/PRC/5 cell lines for total culturable viral assay of treated sewage

Roberto A. Rodríguez, Patricia M. Gundy, Charles P Gerba

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The objective of this study was to compare PLC/PRF/5 and BGM cell lines for use in a total culturable viral assay (TCVA) of treated sewage effluents. Samples were collected before and after chlorination from an activated sludge wastewater treatment plant and from the effluent of a high-rate enhanced flocculation system, followed by UV light disinfection. Cell monolayers were observed for cytopathic effect (CPE) after two passages of 14 days each. Monolayers exhibiting viral CPE were tested for the presence of adenoviruses and enteroviruses by PCR or reverse transcription-PCR. Eight percent of the samples exhibited CPE on BGM cells, and 57% showed CPE on PLC/PRF/5 cells. Only enteroviruses were detected on the BGM cells, while 30% and 52% of the samples were positive for enteroviruses and adenoviruses, respectively, on the PLC/PRF/5 cells. Thirty percent of the samples were positive for both adenoviruses and enteroviruses in chlorinated activated sludge effluent. Thirty percent of the samples were positive for adenoviruses in the UV treatment effluent, but no enteroviruses were detected. In conclusion, the PLC/PRF/5 cells were more susceptible than BGM cells to viruses found in treated sewage. The use of BGM cells for TCVA may underestimate viral concentration in sewage effluent samples. The PLC/PRF/5 cells were more susceptible to adenoviruses, which is important in the evaluation of UV disinfection systems because adenoviruses are highly resistant to UV inactivation.

Original languageEnglish (US)
Pages (from-to)2583-2587
Number of pages5
JournalApplied and Environmental Microbiology
Volume74
Issue number9
DOIs
StatePublished - May 2008

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Sewage
sewage
cell lines
assay
Adenoviridae
effluent
Enterovirus
Cell Line
assays
cytopathogenicity
disinfection
cells
activated sludge
effluents
sewage effluent
Disinfection
sampling
chlorination
flocculation
Viral Cytopathogenic Effect

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Comparison of BGM and PLC/PRC/5 cell lines for total culturable viral assay of treated sewage. / Rodríguez, Roberto A.; Gundy, Patricia M.; Gerba, Charles P.

In: Applied and Environmental Microbiology, Vol. 74, No. 9, 05.2008, p. 2583-2587.

Research output: Contribution to journalArticle

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abstract = "The objective of this study was to compare PLC/PRF/5 and BGM cell lines for use in a total culturable viral assay (TCVA) of treated sewage effluents. Samples were collected before and after chlorination from an activated sludge wastewater treatment plant and from the effluent of a high-rate enhanced flocculation system, followed by UV light disinfection. Cell monolayers were observed for cytopathic effect (CPE) after two passages of 14 days each. Monolayers exhibiting viral CPE were tested for the presence of adenoviruses and enteroviruses by PCR or reverse transcription-PCR. Eight percent of the samples exhibited CPE on BGM cells, and 57{\%} showed CPE on PLC/PRF/5 cells. Only enteroviruses were detected on the BGM cells, while 30{\%} and 52{\%} of the samples were positive for enteroviruses and adenoviruses, respectively, on the PLC/PRF/5 cells. Thirty percent of the samples were positive for both adenoviruses and enteroviruses in chlorinated activated sludge effluent. Thirty percent of the samples were positive for adenoviruses in the UV treatment effluent, but no enteroviruses were detected. In conclusion, the PLC/PRF/5 cells were more susceptible than BGM cells to viruses found in treated sewage. The use of BGM cells for TCVA may underestimate viral concentration in sewage effluent samples. The PLC/PRF/5 cells were more susceptible to adenoviruses, which is important in the evaluation of UV disinfection systems because adenoviruses are highly resistant to UV inactivation.",
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