Comparison of bilayer and monolayer properties of phospholipid systems containing dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylinositol

Heidi - Mansour, Da Sheng Wang, Ching Shih Chen, George Zografi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Bilayer and monolayer phase behavior at the air-water interface of dipalmitoylphosphatidylglycerol (DPPG), dipalmitoylphosphatidylinositol (DPPI), and their binary mixtures with dipalmitoylphosphatidylcholine (DPPC), in the context of their possible roles in lung surfactant function, have been systematically compared. Surface properties, as a function of temperature and composition, were evaluated and analyzed by measuring equilibrium surface pressure-area isotherms (π-A), equilibrium monolayer collapse pressure (πc), and minimum area per molecule at the monolayer collapse pressure (Ac) and by direct observation using fluorescence microscopy. Bilayer properties were evaluated by measuring the main bilayer phase transition temperature (Tm) and the bilayer equilibrium spreading pressure (πe) as a function of temperature and composition. Through thermodynamic analysis, it was found that DPPC/DPPG mix ideally in both the monolayer and bilayer states, whereas DPPC/DPPI (further supported with DPPC/Soy PI) are phase-separated with partial miscibility in bilayers and miscible with very significant positive deviations from ideality in monolayers. This behavior is attributed to the distinct differences between PG and PI headgroup properties as reflected in their physical size, state of hydration, and possible conformational flexibility, despite identical net negative charge and identical acyl chain headgroup properties. PC and PG exhibit very similar headgroup properties, which allow the dipalmitoyl chain properties to dominate over headgroup effects in bilayers and monolayers. In contrast, the unique PI headgroup properties dominate over the dipalmitoyl chain effects giving rise to very different surface and bilayer phase behavior.

Original languageEnglish (US)
Pages (from-to)6622-6632
Number of pages11
JournalLangmuir
Volume17
Issue number21
DOIs
StatePublished - Oct 16 2001
Externally publishedYes

Fingerprint

Phospholipids
1,2-Dipalmitoylphosphatidylcholine
Monolayers
Phase behavior
Fluorescence microscopy
Binary mixtures
Chemical analysis
Surface-Active Agents
Hydration
lungs
surface properties
binary mixtures
Superconducting transition temperature
Surface properties
Isotherms
hydration
1,2-dipalmitoylphosphatidylglycerol
flexibility
isotherms
Surface active agents

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Comparison of bilayer and monolayer properties of phospholipid systems containing dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylinositol. / Mansour, Heidi -; Wang, Da Sheng; Chen, Ching Shih; Zografi, George.

In: Langmuir, Vol. 17, No. 21, 16.10.2001, p. 6622-6632.

Research output: Contribution to journalArticle

@article{6850108197fa45e1bbbb3d5dc9b29735,
title = "Comparison of bilayer and monolayer properties of phospholipid systems containing dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylinositol",
abstract = "Bilayer and monolayer phase behavior at the air-water interface of dipalmitoylphosphatidylglycerol (DPPG), dipalmitoylphosphatidylinositol (DPPI), and their binary mixtures with dipalmitoylphosphatidylcholine (DPPC), in the context of their possible roles in lung surfactant function, have been systematically compared. Surface properties, as a function of temperature and composition, were evaluated and analyzed by measuring equilibrium surface pressure-area isotherms (π-A), equilibrium monolayer collapse pressure (πc), and minimum area per molecule at the monolayer collapse pressure (Ac) and by direct observation using fluorescence microscopy. Bilayer properties were evaluated by measuring the main bilayer phase transition temperature (Tm) and the bilayer equilibrium spreading pressure (πe) as a function of temperature and composition. Through thermodynamic analysis, it was found that DPPC/DPPG mix ideally in both the monolayer and bilayer states, whereas DPPC/DPPI (further supported with DPPC/Soy PI) are phase-separated with partial miscibility in bilayers and miscible with very significant positive deviations from ideality in monolayers. This behavior is attributed to the distinct differences between PG and PI headgroup properties as reflected in their physical size, state of hydration, and possible conformational flexibility, despite identical net negative charge and identical acyl chain headgroup properties. PC and PG exhibit very similar headgroup properties, which allow the dipalmitoyl chain properties to dominate over headgroup effects in bilayers and monolayers. In contrast, the unique PI headgroup properties dominate over the dipalmitoyl chain effects giving rise to very different surface and bilayer phase behavior.",
author = "Mansour, {Heidi -} and Wang, {Da Sheng} and Chen, {Ching Shih} and George Zografi",
year = "2001",
month = "10",
day = "16",
doi = "10.1021/la0108454",
language = "English (US)",
volume = "17",
pages = "6622--6632",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Comparison of bilayer and monolayer properties of phospholipid systems containing dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylinositol

AU - Mansour, Heidi -

AU - Wang, Da Sheng

AU - Chen, Ching Shih

AU - Zografi, George

PY - 2001/10/16

Y1 - 2001/10/16

N2 - Bilayer and monolayer phase behavior at the air-water interface of dipalmitoylphosphatidylglycerol (DPPG), dipalmitoylphosphatidylinositol (DPPI), and their binary mixtures with dipalmitoylphosphatidylcholine (DPPC), in the context of their possible roles in lung surfactant function, have been systematically compared. Surface properties, as a function of temperature and composition, were evaluated and analyzed by measuring equilibrium surface pressure-area isotherms (π-A), equilibrium monolayer collapse pressure (πc), and minimum area per molecule at the monolayer collapse pressure (Ac) and by direct observation using fluorescence microscopy. Bilayer properties were evaluated by measuring the main bilayer phase transition temperature (Tm) and the bilayer equilibrium spreading pressure (πe) as a function of temperature and composition. Through thermodynamic analysis, it was found that DPPC/DPPG mix ideally in both the monolayer and bilayer states, whereas DPPC/DPPI (further supported with DPPC/Soy PI) are phase-separated with partial miscibility in bilayers and miscible with very significant positive deviations from ideality in monolayers. This behavior is attributed to the distinct differences between PG and PI headgroup properties as reflected in their physical size, state of hydration, and possible conformational flexibility, despite identical net negative charge and identical acyl chain headgroup properties. PC and PG exhibit very similar headgroup properties, which allow the dipalmitoyl chain properties to dominate over headgroup effects in bilayers and monolayers. In contrast, the unique PI headgroup properties dominate over the dipalmitoyl chain effects giving rise to very different surface and bilayer phase behavior.

AB - Bilayer and monolayer phase behavior at the air-water interface of dipalmitoylphosphatidylglycerol (DPPG), dipalmitoylphosphatidylinositol (DPPI), and their binary mixtures with dipalmitoylphosphatidylcholine (DPPC), in the context of their possible roles in lung surfactant function, have been systematically compared. Surface properties, as a function of temperature and composition, were evaluated and analyzed by measuring equilibrium surface pressure-area isotherms (π-A), equilibrium monolayer collapse pressure (πc), and minimum area per molecule at the monolayer collapse pressure (Ac) and by direct observation using fluorescence microscopy. Bilayer properties were evaluated by measuring the main bilayer phase transition temperature (Tm) and the bilayer equilibrium spreading pressure (πe) as a function of temperature and composition. Through thermodynamic analysis, it was found that DPPC/DPPG mix ideally in both the monolayer and bilayer states, whereas DPPC/DPPI (further supported with DPPC/Soy PI) are phase-separated with partial miscibility in bilayers and miscible with very significant positive deviations from ideality in monolayers. This behavior is attributed to the distinct differences between PG and PI headgroup properties as reflected in their physical size, state of hydration, and possible conformational flexibility, despite identical net negative charge and identical acyl chain headgroup properties. PC and PG exhibit very similar headgroup properties, which allow the dipalmitoyl chain properties to dominate over headgroup effects in bilayers and monolayers. In contrast, the unique PI headgroup properties dominate over the dipalmitoyl chain effects giving rise to very different surface and bilayer phase behavior.

UR - http://www.scopus.com/inward/record.url?scp=0035900141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035900141&partnerID=8YFLogxK

U2 - 10.1021/la0108454

DO - 10.1021/la0108454

M3 - Article

VL - 17

SP - 6622

EP - 6632

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 21

ER -