Comparison of control of Listeria by nitric oxide redox chemistry from murine macrophages and NO donors

Insights into Listeriocidal activity of oxidative and nitrosative stress

Ryohei Ogawa, Roberto Pacelli, Michael G. Espey, Katrina M Miranda, Norman Friedman, SungMee Kim, George Cox, James B. Mitchell, David A. Wink, Angelo Russo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The physiological function of nitric oxide (NO) in the defense against pathogens is multifaceted. The exact chemistry by which NO combats intracellular pathogens such as Listeria monocytogenes is yet unresolved. We examined the effects of NO exposure, either delivered by NO donors or generated in situ within ANA-1 murine macrophages, on L. monocytogenes growth. Production of NO by the two NONOate compounds PAPA/NO (NH2(C3H6) (N[N(O)NO]C3H7)) and DEA/NO (Na(C2H5)2N[N(O)NO]) resulted in L. monocytogenes cytostasis with minimal cytotoxicity. Reactive oxygen species generated from xanthine oxidase/hypoxanthine were neither bactericidal nor cytostatic and did not alter the action of NO. L. monocytogenes growth was also suppressed upon internalization into ANA-1 murine macrophages primed with interferon-γ (INF-γ) + tumor necrosis factor-α (TNF-α or INF-γ + lipid polysaccharide (LPS). Growth suppression correlated with nitrite formation and nitrosation of 2,3-diaminonaphthalene elicited by stimulated murine macrophages. This nitrosative chemistry was not dependent upon nor mediated by interaction with reactive oxygen species (ROS), but resulted solely from NO and intermediates related to nitrosative stress. The role of nitrosation in controlling L. monocytogenes was further examined by monitoring the effects of exposure to NO on an important virulence factor, Listeriolysin O, which was inhibited under nitrosative conditions. These results suggest that nitrosative stress mediated by macrophages is an important component of the immunological arsenal in controlling L. monocytogenes infections.

Original languageEnglish (US)
Pages (from-to)268-276
Number of pages9
JournalFree Radical Biology and Medicine
Volume30
Issue number3
DOIs
StatePublished - Feb 1 2001
Externally publishedYes

Fingerprint

Listeria
Nitric Oxide Donors
Macrophages
Oxidation-Reduction
Nitric Oxide
Oxidative Stress
Listeria monocytogenes
Nitrosation
Pathogens
Interferons
Reactive Oxygen Species
Growth
Arsenals
Listeriosis
Xanthine Oxidase
Cytostatic Agents
Virulence Factors
Cytotoxicity
Nitrites
Polysaccharides

Keywords

  • Free radicals
  • Listeria
  • Nitrosation stress
  • NO

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Comparison of control of Listeria by nitric oxide redox chemistry from murine macrophages and NO donors : Insights into Listeriocidal activity of oxidative and nitrosative stress. / Ogawa, Ryohei; Pacelli, Roberto; Espey, Michael G.; Miranda, Katrina M; Friedman, Norman; Kim, SungMee; Cox, George; Mitchell, James B.; Wink, David A.; Russo, Angelo.

In: Free Radical Biology and Medicine, Vol. 30, No. 3, 01.02.2001, p. 268-276.

Research output: Contribution to journalArticle

Ogawa, Ryohei ; Pacelli, Roberto ; Espey, Michael G. ; Miranda, Katrina M ; Friedman, Norman ; Kim, SungMee ; Cox, George ; Mitchell, James B. ; Wink, David A. ; Russo, Angelo. / Comparison of control of Listeria by nitric oxide redox chemistry from murine macrophages and NO donors : Insights into Listeriocidal activity of oxidative and nitrosative stress. In: Free Radical Biology and Medicine. 2001 ; Vol. 30, No. 3. pp. 268-276.
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