Comparison of DNA extraction methods for PCR amplification of mitochondrial cytochrome c oxidase subunit II (COII) DNA from primate fecal samples

Christopher A. Whittier, Arun K. Dhar, Chip Stem, Jane Goodall, Acacia Alcivar-Warren

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Mitochondrial COII DNA was amplified by PCR from total DNA extracted from field collected primate fecal samples (n = 24) which had been stored without refrigeration for over 30 days. High molecular weight DNA total DNA was obtained from samples stored in 70% (v/v) ethanol, SDS lysis buffer (LB) and guanidine isothiocyanate buffer (GTB) than from samples stored in 10% formalin. Fecal DNA quality and COII amplification varied according to storage solution (formalin, ethanol, LB and GTB), extraction method (LB-based and GTB-based) and primate species (chimpanzee, baboon, human). It is recommended that fecal samples be collected in LB for DNA analysis. However, GTB-based protocols are suitable when total RNA is needed for epidemiological studies of viral diseases or gene expression analysis.

Original languageEnglish (US)
Pages (from-to)771-779
Number of pages9
JournalBiotechnology Techniques
Volume13
Issue number11
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Baboon
  • COII
  • Chimpanzee
  • DNA
  • Fecal
  • Human

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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