Comparison of four Taura syndrome virus (TSV) isolates in oral challenge studies with Litopenaeus vannamei unselected or selected for resistance to TSV

Thinnarat Srisuvan, Brenda L. Noble, Paul J. Schofield, Donald V Lightner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Taura syndrome virus (TSV) infection in TSV-resistant (TSR) and TSV-susceptible (Kona) Litopenaeus vannamei (also called Penaeus vannamei) was investigated using histology, in situ hybridization (ISH), conventional reverse transcription polymerase chain reaction (RT-PCR) assays, and SYBR-Green real-time RT-PCR analysis. The shrimp were challenged by feeding with minced tissues of L. vannamei infected with 4 genotypic variants of TSV (Bz01, Th04, UsHi94, and Ve05). Survival probabilities of TSR shrimp were higher than those for Kona shrimp with all 4 variants. Th04, UsHi94, and Ve05 gave no Taura syndrome lesions with TSR shrimp. In contrast, TSR shrimp challenged with Bz01 and Kona shrimp with all 4 TSV variants exhibited severe necrosis of cuticular epithelial cells and lymphoid organ spheroids, indicative of acute and chronic phases of TSV infection, respectively. TSV was not detected by RT-PCR in TSR shrimp infected with Th04, UsHi94, and Ve05, or in Kona shrimp infected with Ve05 but was detected in TSR shrimp infected with Bz01 and in Kona shrimp infected with Bz01, Th04, and UsHi94. Real-time RT-PCR revealed that mean TSV copy numbers in TSR shrimp infected with Bz01, Th04, and UsHi94 were significantly (p < 0.0005) lower than those in Kona shrimp. In contrast, mean TSV copy numbers in TSR and Kona shrimp infected with Ve05 were not significantly different (p > 0.4). The results show that TSR L. vannamei are susceptible to infection but give high survival rates following challenge by all 4 variants of TSV.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalDiseases of Aquatic Organisms
Volume71
Issue number1
StatePublished - Jul 11 2006

Fingerprint

Taura syndrome virus
Litopenaeus vannamei
mouth
virus
shrimp
polymerase chain reaction
reverse transcriptase polymerase chain reaction
quantitative polymerase chain reaction
comparison
histology
lesion
biological resistance
lesions (animal)
infection
in situ hybridization
assay

Keywords

  • Litopenaeus vannamei
  • Taura syndrome virus
  • TSV
  • TSV variants

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Comparison of four Taura syndrome virus (TSV) isolates in oral challenge studies with Litopenaeus vannamei unselected or selected for resistance to TSV. / Srisuvan, Thinnarat; Noble, Brenda L.; Schofield, Paul J.; Lightner, Donald V.

In: Diseases of Aquatic Organisms, Vol. 71, No. 1, 11.07.2006, p. 1-10.

Research output: Contribution to journalArticle

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