Comparison of in vitro virulence factors of campylobacter jejuni to in vivo lesion production

Bibiana Law, Sarah M. Adriance, Lynn A. Joens

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Campylobacter jejuni is recognized as one of the most common agents of food-borne bacterial gastroenteritis in humans. Previous work has shown that C. jejuni isolates vary in their ability to invade and survive in laboratory grown cells. The correlation of these assays to actual lesion development in the hosts has not been determined. Therefore, this study aims to define the relationship between in vitro and in vivo assays for determining the virulence of C. jejuni isolates. Fifty-nine C. jejuni poultry isolates were analyzed in invasion and macrophage assays, and five isolates showing different invasion and survival abilities were examined for pathogenicity in the piglet model. All five isolates examined in the piglet model induced diarrhea without the presence of blood. Four of the five isolates produced microscopic lesions in piglets consisting of mucosal congestion, villous degeneration, and epithelial cell erosion. These studies imply that invasion or macrophage survival had little effect on the production of lesions typical of those noted in patients with campylobacteriosis. The most surprising finding was that isolates that produced a fluid exudate in piglets failed to invade epithelial cells. It is not known if the production of this fluid exudate is related to the production of a toxin(s) by C. jejuni. More work on the identification of the gene expressing this virulence factor is needed to confirm that this is indeed a putative toxin produced by C. jejuni.

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalFoodborne Pathogens and Disease
Volume6
Issue number3
DOIs
StatePublished - Apr 1 2009

Fingerprint

Campylobacter jejuni
Virulence Factors
lesions (animal)
virulence
piglets
Exudates and Transudates
Virulence
macrophages
epithelial cells
assays
toxins
Epithelial Cells
Macrophages
campylobacteriosis
gastroenteritis
Gastroenteritis
Poultry
In Vitro Techniques
Diarrhea
diarrhea

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Comparison of in vitro virulence factors of campylobacter jejuni to in vivo lesion production. / Law, Bibiana; Adriance, Sarah M.; Joens, Lynn A.

In: Foodborne Pathogens and Disease, Vol. 6, No. 3, 01.04.2009, p. 377-385.

Research output: Contribution to journalArticle

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