Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization

C. E. Enriquez, M. Abbaszadegan, I. L. Pepper, K. J. Richardson, A. B. Margolin, C. P. Gerba

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalWater Science and Technology
Volume27
Issue number3-4
StatePublished - Jun 15 1993
EventProceedings of the 16th Biennial Conference and Exposition of the International Association on Water Pollution Research and Control - Washington, DC, USA
Duration: May 24 1992May 30 1992

Keywords

  • Nucleic acid hybridization
  • Poliovirus
  • Viral detection

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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    Enriquez, C. E., Abbaszadegan, M., Pepper, I. L., Richardson, K. J., Margolin, A. B., & Gerba, C. P. (1993). Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization. Water Science and Technology, 27(3-4), 315-319.