Comparison of rat sensory behavioral tasks to detect somatosensory morbidity after diffuse brain-injury

Annastazia Ellouise Learoyd, Jonathan Lifshitz

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Brain injury disrupts neuronal circuits, impacting neurological function. Selective and sensitive behavioral tests are required to explore neurological dysfunction, recovery and potential therapy. Previously we reported that the Whisker Nuisance Task (WNT), where whiskers are manually stimulated in an open field, shows sensory sensitivity in diffuse brain-injured rats. To further explore this somatosensory morbidity, we evaluated three additional whisker-dependent tasks: Gap Cross Test, a novel Angle Entrance Task and Whisker Guided Exploration Task. Brain-injured (n= 11) and sham (n= 8) rats were tested before midline fluid percussion brain injury (moderate: 2.0. atm) and 1 and 4 weeks after injury. For the WNT, we confirmed that brain-injured rats develop significant sensory sensitivity to whisker stimulation over 28 days. In the Gap Cross Test, where rats cross progressively larger elevated gaps, we found that animals were inconsistent in crossable distance regardless of injury. In the Angle Entrance Task, where rats enter 30°, 40°, 50° or 80° corners, rats performed consistently regardless of injury. In the Whisker Guided Exploration Task, where rats voluntarily explore an oval circuit, we identified significant decreases in the number of rears and reversals and changes in the predominant location (injured rats spend more time in the inside of the turn compared to the outside) after injury and increased thigmotaxis after sham and brain-injury. Both the WNT and Whisker Guided Exploration Task show injury-induced somatosensory behavioral morbidity; however, the WNT remains more sensitive in detecting brain injury, possibly due to imposed whisker stimulation that elicits agitation similar to the human condition.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalBehavioural Brain Research
Volume226
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • Angle Entrance Task
  • Diffuse brain injury
  • Gap Cross Test
  • Whisker Guided Exploration Task
  • Whisker Nuisance Task

ASJC Scopus subject areas

  • Behavioral Neuroscience

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