Comparison of Reproductive Function in Female TgMISIIR-TAg Transgenic and Wildtype C57BL/6 Mice

Patricia B Hoyer, Photini F. Rice, Caitlin C. Howard, Jennifer W. Koevary, Joceline P. Dominguez Cooks, Gabrielle V. Hutchens, Setsuko K Chambers, Zelieann R Craig, Denise C. Connolly, Jennifer K Barton

Research output: Contribution to journalArticle

Abstract

Transgenic TgMISIIR-TAg (TAg) mice express the oncogenic virus SV40 in Mullerian epithelial cells. Female TAg mice spontaneously develop epithelial ovarian carcinoma, the most common type of ovarian cancer in women. Female TAg mice are infertile, but the reason has not been determined. We therefore investigated whether female TAg mice undergo puberty, demonstrate follicular development, maintain regular cycles, and ovulate. Ovarian cancers in women commonly develop after menopause. The occupational chemical 4-vinylcyclohexene diepoxide (VCD) accelerates follicle degeneration in the ovaries of rats and mice, causing early ovarian failure. We therefore used VCD dosing of mice to develop an animal model for menopause. The purpose of this study was to characterize reproductive parameters in female TAg mice and to investigate whether the onset of ovarian failure due VCD dosing differed between female TAg and WT C57BL/6 mice. As in WT female mice, TAg female mice underwent puberty (vaginal opening) and developed cyclicity in patterns that were similar between the groups. Vehicle-only TAg mice had fewer ovulations (numbers of corpora lutea) than WT animals. VCD exposure delayed the onset of puberty (day of first estrus) in TAg as compared with WT mice. Morphologic evaluation of ovaries revealed many more degenerating follicles in TAg mice than WT mice, and more VCD-dosed TAg mice were in ovarian failure than VCD-dosed WT mice. These results suggest that despite showing similar onset of sexual maturation, TAg mice have increased follicular degeneration and fewer ovulations than WT. These features may contribute to the inability of female TAg mice to reproduce.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalComparative medicine
Volume69
Issue number1
DOIs
StatePublished - Feb 1 2019

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Inbred C57BL Mouse
genetically modified organisms
mice
Oncogenic viruses
Animals
puberty
4-vinyl-1-cyclohexene dioxide
Rats
menopause
ovarian neoplasms
Puberty
Menopause
Ovulation
Ovarian Neoplasms
Ovary
ovulation
oncogenic viruses
Delayed Puberty
Sexual Maturation
Oncogenic Viruses

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Comparison of Reproductive Function in Female TgMISIIR-TAg Transgenic and Wildtype C57BL/6 Mice. / Hoyer, Patricia B; Rice, Photini F.; Howard, Caitlin C.; Koevary, Jennifer W.; Dominguez Cooks, Joceline P.; Hutchens, Gabrielle V.; Chambers, Setsuko K; Craig, Zelieann R; Connolly, Denise C.; Barton, Jennifer K.

In: Comparative medicine, Vol. 69, No. 1, 01.02.2019, p. 16-21.

Research output: Contribution to journalArticle

Hoyer, Patricia B ; Rice, Photini F. ; Howard, Caitlin C. ; Koevary, Jennifer W. ; Dominguez Cooks, Joceline P. ; Hutchens, Gabrielle V. ; Chambers, Setsuko K ; Craig, Zelieann R ; Connolly, Denise C. ; Barton, Jennifer K. / Comparison of Reproductive Function in Female TgMISIIR-TAg Transgenic and Wildtype C57BL/6 Mice. In: Comparative medicine. 2019 ; Vol. 69, No. 1. pp. 16-21.
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