Comparison of Sarns 3M heparin bonded to Duraflo II and control circuits in a porcine model

Macro- and microanalysis of thrombi accumulation in circuit arterial filters

Douglas F Larson, David Arzouman, Leigh Kleinert, Vangie Patula, Stuart Williams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Heparin-bonded perfusion circuits have been reported to reduce the thrombus formation during various levels of systemic heparinization. The goal of this study was to compare the efficacy of thrombo-resistance of the Sarns 3M heparin-bonded circuit to Baxter Duraflo II and untreated control in a porcine model. Fifteen Yorkshire pigs (60-65 kg) were anesthetized, heparinized with 3000 IU, intravenously (i.v.) and surgically cannulated with an internal jugular outflow and a femoral vein inflow. All circuits consisted of a 22-Fr venous cannula, centrifugal pump, arterial filter, an 18-Fr cannula for return and connected with equal lengths of 3/8' polyvinyl chloride tubing. The flows were maintained at 2.0 l/min for 4 h. Thrombus formation in filter samples were morphometrically analyzed through macro-densitometry, light microscopy, and scanning electron microscopy (SEM). Our findings revealed that the 3M circuit had significantly less gross thrombus (p < 0.001), 66% and 84% less microscopic thrombi and fivefold less SEM-measured aggregates (p = 0.03) compared to the Duraflo II and uncoated groups. This study demonstrated that the 3M heparin-bonded circuit had significantly reduced the formation of micro- and macro-thrombi in the minimally heparinized pig model compared to the Duraflo II and untreated control circuits.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalPerfusion
Volume15
Issue number1
StatePublished - 2000

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Microanalysis
Macros
Heparin
Thrombosis
Swine
Networks (circuits)
Electron Scanning Microscopy
Scanning electron microscopy
Femoral Vein
Group
Densitometry
Centrifugal pumps
Tubing
Polyvinyl Chloride
Polyvinyl chlorides
Optical microscopy
Microscopy
Neck
Perfusion
Light

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Comparison of Sarns 3M heparin bonded to Duraflo II and control circuits in a porcine model : Macro- and microanalysis of thrombi accumulation in circuit arterial filters. / Larson, Douglas F; Arzouman, David; Kleinert, Leigh; Patula, Vangie; Williams, Stuart.

In: Perfusion, Vol. 15, No. 1, 2000, p. 13-20.

Research output: Contribution to journalArticle

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