Compartmentation of carbohydrate metabolism in vascular smooth muscle: evidence for at least two functionally independent pools of glucose 6-phosphate

Ronald M. Lynch, Richard J. Paul

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Previous work has indicated that there are at least two functionally independent Embden-Meyerhof pathways within the vascular smooth muscle of porcine carotid artery. We tested this hypothesis by analyzing the isotopic equilibrium between medium glucose and intracellular glucose 6-phosphate under basal conditions and after 30 min of mechanical activation, during which time the rate of glycogenolysis has been found to be substantial. Under basal conditions, the specific activity of glucose 6-phosphate equilibrated to a level which was not in isotopic equilibrium with medium glucose suggesting that there is a significant pool of glucose 6-phosphate which is not readily accessible to medium glucose. After 15 min of mechanical activation, the specific activity of intracellular glucose 6-phosphate was found to decrease significantly from its apparent steady-state distribution, indicating that glycogen was likely to be a significant source for glucose 6-phosphate. Since the specific activity of lactate was unaltered from its equilibrium distribution under similar stimulus conditions, these findings substantiate the existence of at least two independent pools of glucose 6-phosphate.

Original languageEnglish (US)
Pages (from-to)315-318
Number of pages4
JournalBBA - Molecular Cell Research
Volume887
Issue number3
DOIs
StatePublished - Aug 1 1986
Externally publishedYes

Keywords

  • (Vascular smooth muscle)
  • Carbohydrate metabolism
  • Compartmentation
  • Glucose 6-phosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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