Competitive engraftment of adult and fetal murine hematopoietic progenitor cells following in utero transplantation

D. R. Archer, C. W. Turner, P. L. Turner, L. E. Hester, Andrew M Yeager, W. H. Fleming

Research output: Contribution to journalArticle

Abstract

The engraftment potential of adult bone marrow and fetal liver hematopoietic progenitor cells was compared following transplantation into 13.5 day gestation NOD/LtSz-scW/J (Ly-5.2) mouse embryos. Donor cells were prepared from mice expressing Ly-5.1 alone (adult bone marrow; C57B1/6), or those co-expressing Ly-5.1 and Ly-5.2 (fetal liver; C57B1/6J × B6.SJL). Cells from fetal liver and bone marrow (6 months of age) were depleted of lineage-positive cells using immunomagnetic beads. Recipient embryos were injected with a maximum of 3ul of a solution containing equal numbers of fetal and adult cells (final concentration 4×105 cells/ul). Four weeks after birth recipients were analyzed. Recipients were considered chimeric if > 0.5% of the cells in peripheral blood (PB) bone marrow (BM). or spleen were of donor origin. Ninety-two percent of recipients were chimeric in the spleen and 77% in the PB whilst only 38% were chimeric in the BM. Similar patterns of engraftment were found in both PB and spleen. Fetal liver-derived cells were found in 100% of the chimeric animals, whereas adult bone marrow engrafted in 60% of chimeric mice. The frequency of donor cells derived donor from fetal liver was 5-10 fold greater than that contributed from adult donor cells. Donor myeloid cells were present in the PB although lymphoid (T- and B) cells predominated in both PB and spleen. The bone marrow of engrafted recipients typically showed low levels of donor cells between 0.5 and 1%. These results clearly demonstrate that fetal liver-derived hematopoietic progenitor cells have a higher engraftment potential on a per cell basis than adult BM-derived progenitor cells. Ongoing secondary transplant studies will define the frequency of transplantable hematopoietic stem cells derived from fetal live and adult BM.

Original languageEnglish (US)
Pages (from-to)741
Number of pages1
JournalExperimental Hematology
Volume25
Issue number8
StatePublished - 1997
Externally publishedYes

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Hematopoietic Stem Cells
Transplantation
Bone Marrow
Tissue Donors
Liver
Spleen
Embryonic Structures
Myeloid Cells
B-Lymphocytes
Stem Cells
Parturition
Transplants
Pregnancy

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Competitive engraftment of adult and fetal murine hematopoietic progenitor cells following in utero transplantation. / Archer, D. R.; Turner, C. W.; Turner, P. L.; Hester, L. E.; Yeager, Andrew M; Fleming, W. H.

In: Experimental Hematology, Vol. 25, No. 8, 1997, p. 741.

Research output: Contribution to journalArticle

Archer, D. R. ; Turner, C. W. ; Turner, P. L. ; Hester, L. E. ; Yeager, Andrew M ; Fleming, W. H. / Competitive engraftment of adult and fetal murine hematopoietic progenitor cells following in utero transplantation. In: Experimental Hematology. 1997 ; Vol. 25, No. 8. pp. 741.
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abstract = "The engraftment potential of adult bone marrow and fetal liver hematopoietic progenitor cells was compared following transplantation into 13.5 day gestation NOD/LtSz-scW/J (Ly-5.2) mouse embryos. Donor cells were prepared from mice expressing Ly-5.1 alone (adult bone marrow; C57B1/6), or those co-expressing Ly-5.1 and Ly-5.2 (fetal liver; C57B1/6J × B6.SJL). Cells from fetal liver and bone marrow (6 months of age) were depleted of lineage-positive cells using immunomagnetic beads. Recipient embryos were injected with a maximum of 3ul of a solution containing equal numbers of fetal and adult cells (final concentration 4×105 cells/ul). Four weeks after birth recipients were analyzed. Recipients were considered chimeric if > 0.5{\%} of the cells in peripheral blood (PB) bone marrow (BM). or spleen were of donor origin. Ninety-two percent of recipients were chimeric in the spleen and 77{\%} in the PB whilst only 38{\%} were chimeric in the BM. Similar patterns of engraftment were found in both PB and spleen. Fetal liver-derived cells were found in 100{\%} of the chimeric animals, whereas adult bone marrow engrafted in 60{\%} of chimeric mice. The frequency of donor cells derived donor from fetal liver was 5-10 fold greater than that contributed from adult donor cells. Donor myeloid cells were present in the PB although lymphoid (T- and B) cells predominated in both PB and spleen. The bone marrow of engrafted recipients typically showed low levels of donor cells between 0.5 and 1{\%}. These results clearly demonstrate that fetal liver-derived hematopoietic progenitor cells have a higher engraftment potential on a per cell basis than adult BM-derived progenitor cells. Ongoing secondary transplant studies will define the frequency of transplantable hematopoietic stem cells derived from fetal live and adult BM.",
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