Competitiveness of KIM 5 and VIKING 1 bean rhizobia

Strain by cultivar interactions

K. L. Josephson, D. P. Bourque, F. A. Bliss, Ian L Pepper

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Competitiveness, which in rhizobia refers to the relative ability of a strain to inject a legume and cause nodule formation in the presence of other strains, is critical to biological N2-fixation. The mechanisms for strain competitiveness are unknown, but are probably affected by biotic and abiotic factors. The competitiveness of two strains of rhizobia (Rhizobium leguminosarum biovar phaseoli) KIM 5 and VIKING 1 that are known to be highly competitive were evaluated when added together as inoculant to 12 bean (Phaseolus vulgaris L.) host cultivars grown in Leonard jars in sterilized sand. Strain identification of bacteria from harvested nodules was determined by fluorescent antibodies. KIM 5 was generally more competitive than VIKING 1, but competitiveness of both strains was highly dependent on strain by cultivar interactions. With Tendergreen, no nodules contained VIKING 1 alone, whereas with Jampa, 46.3 of the nodules contained only VIKING 1. Comparisons of parent cultivar and sib progeny lines showed that even small differences in the host genotype affected the competitiveness of both strains. All symbioses were effective in fixing N2. Based on these data, studies evaluating the competitiveness of rhizobia should utilize several host genotypes to avoid misinterpretations of the competitiveness of a given strain.

Original languageEnglish (US)
Pages (from-to)249-253
Number of pages5
JournalSoil Biology and Biochemistry
Volume23
Issue number3
DOIs
StatePublished - 1991

Fingerprint

Rhizobium leguminosarum bv. phaseoli
Rhizobium leguminosarum
rhizobacterium
competitiveness
cultivar
beans
cultivars
Genotype
Nitrogen Fixation
Phaseolus
Symbiosis
Fabaceae
genotype
Bacteria
Antibodies
biotic factor
symbiosis
antibody
fixation
jars

ASJC Scopus subject areas

  • Soil Science
  • Biochemistry
  • Ecology

Cite this

Competitiveness of KIM 5 and VIKING 1 bean rhizobia : Strain by cultivar interactions. / Josephson, K. L.; Bourque, D. P.; Bliss, F. A.; Pepper, Ian L.

In: Soil Biology and Biochemistry, Vol. 23, No. 3, 1991, p. 249-253.

Research output: Contribution to journalArticle

Josephson, K. L. ; Bourque, D. P. ; Bliss, F. A. ; Pepper, Ian L. / Competitiveness of KIM 5 and VIKING 1 bean rhizobia : Strain by cultivar interactions. In: Soil Biology and Biochemistry. 1991 ; Vol. 23, No. 3. pp. 249-253.
@article{7f9d34bb1c964ab585dbb2566899a26c,
title = "Competitiveness of KIM 5 and VIKING 1 bean rhizobia: Strain by cultivar interactions",
abstract = "Competitiveness, which in rhizobia refers to the relative ability of a strain to inject a legume and cause nodule formation in the presence of other strains, is critical to biological N2-fixation. The mechanisms for strain competitiveness are unknown, but are probably affected by biotic and abiotic factors. The competitiveness of two strains of rhizobia (Rhizobium leguminosarum biovar phaseoli) KIM 5 and VIKING 1 that are known to be highly competitive were evaluated when added together as inoculant to 12 bean (Phaseolus vulgaris L.) host cultivars grown in Leonard jars in sterilized sand. Strain identification of bacteria from harvested nodules was determined by fluorescent antibodies. KIM 5 was generally more competitive than VIKING 1, but competitiveness of both strains was highly dependent on strain by cultivar interactions. With Tendergreen, no nodules contained VIKING 1 alone, whereas with Jampa, 46.3 of the nodules contained only VIKING 1. Comparisons of parent cultivar and sib progeny lines showed that even small differences in the host genotype affected the competitiveness of both strains. All symbioses were effective in fixing N2. Based on these data, studies evaluating the competitiveness of rhizobia should utilize several host genotypes to avoid misinterpretations of the competitiveness of a given strain.",
author = "Josephson, {K. L.} and Bourque, {D. P.} and Bliss, {F. A.} and Pepper, {Ian L}",
year = "1991",
doi = "10.1016/0038-0717(91)90060-W",
language = "English (US)",
volume = "23",
pages = "249--253",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Competitiveness of KIM 5 and VIKING 1 bean rhizobia

T2 - Strain by cultivar interactions

AU - Josephson, K. L.

AU - Bourque, D. P.

AU - Bliss, F. A.

AU - Pepper, Ian L

PY - 1991

Y1 - 1991

N2 - Competitiveness, which in rhizobia refers to the relative ability of a strain to inject a legume and cause nodule formation in the presence of other strains, is critical to biological N2-fixation. The mechanisms for strain competitiveness are unknown, but are probably affected by biotic and abiotic factors. The competitiveness of two strains of rhizobia (Rhizobium leguminosarum biovar phaseoli) KIM 5 and VIKING 1 that are known to be highly competitive were evaluated when added together as inoculant to 12 bean (Phaseolus vulgaris L.) host cultivars grown in Leonard jars in sterilized sand. Strain identification of bacteria from harvested nodules was determined by fluorescent antibodies. KIM 5 was generally more competitive than VIKING 1, but competitiveness of both strains was highly dependent on strain by cultivar interactions. With Tendergreen, no nodules contained VIKING 1 alone, whereas with Jampa, 46.3 of the nodules contained only VIKING 1. Comparisons of parent cultivar and sib progeny lines showed that even small differences in the host genotype affected the competitiveness of both strains. All symbioses were effective in fixing N2. Based on these data, studies evaluating the competitiveness of rhizobia should utilize several host genotypes to avoid misinterpretations of the competitiveness of a given strain.

AB - Competitiveness, which in rhizobia refers to the relative ability of a strain to inject a legume and cause nodule formation in the presence of other strains, is critical to biological N2-fixation. The mechanisms for strain competitiveness are unknown, but are probably affected by biotic and abiotic factors. The competitiveness of two strains of rhizobia (Rhizobium leguminosarum biovar phaseoli) KIM 5 and VIKING 1 that are known to be highly competitive were evaluated when added together as inoculant to 12 bean (Phaseolus vulgaris L.) host cultivars grown in Leonard jars in sterilized sand. Strain identification of bacteria from harvested nodules was determined by fluorescent antibodies. KIM 5 was generally more competitive than VIKING 1, but competitiveness of both strains was highly dependent on strain by cultivar interactions. With Tendergreen, no nodules contained VIKING 1 alone, whereas with Jampa, 46.3 of the nodules contained only VIKING 1. Comparisons of parent cultivar and sib progeny lines showed that even small differences in the host genotype affected the competitiveness of both strains. All symbioses were effective in fixing N2. Based on these data, studies evaluating the competitiveness of rhizobia should utilize several host genotypes to avoid misinterpretations of the competitiveness of a given strain.

UR - http://www.scopus.com/inward/record.url?scp=3042767121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042767121&partnerID=8YFLogxK

U2 - 10.1016/0038-0717(91)90060-W

DO - 10.1016/0038-0717(91)90060-W

M3 - Article

VL - 23

SP - 249

EP - 253

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 3

ER -