Complement receptors on normal human lymphocytes containing parallel tubular arrays

C. M. Payne, Raymond B Nagle

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Membrane complement receptors have been identified on a subpopulation of normal lymphocytes containing cytoplasmic inclusions called parallel tubular arrays (PTA) using two different rosetting techniques. The first technique utilizes as indicator cells erythrocytes that were coated with complement by the classic pathway of complement activation (EAC rosettes). The second technique utilizes as indicator cells Salmonella typhi, which were coated with complement by the alternate pathway of complement activation (FBC rosettes). In the latter technique, lipopolysaccharide material in the bacterial cell wall directly activates complement without the use of a sensitizing antibody. This eliminates binding of marker particles by lymphocytes having Fc receptors. The presence of PTA lymphocytes at the center of EAC rosettes and FBC rosettes was demonstrated by electron microscopy, indicating that the PTA lymphocyte has a complement receptor. Examination of FBC rosettes revealed that the adherent complement-coated bacteria but not the complement-inactivated bacteria. These phagocytic cells were placed in the lymphocytic series instead of the monocytic series by virture of complete lack of endogenous peroxidase activity.

Original languageEnglish (US)
Pages (from-to)645-666
Number of pages22
JournalAmerican Journal of Pathology
Volume99
Issue number3
StatePublished - 1980

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Complement Receptors
Complement Activation
Lymphocytes
Bacteria
Salmonella typhi
Fc Receptors
Inclusion Bodies
Lymphocyte Subsets
Phagocytes
Cell Wall
Peroxidase
Lipopolysaccharides
Electron Microscopy
Erythrocytes
Membranes
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Complement receptors on normal human lymphocytes containing parallel tubular arrays. / Payne, C. M.; Nagle, Raymond B.

In: American Journal of Pathology, Vol. 99, No. 3, 1980, p. 645-666.

Research output: Contribution to journalArticle

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