Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5

Debarati Paul, Susan M. Bridges, Shane C Burgess, Yoginder S. Dandass, Mark L. Lawrence

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium capable of utilizing CO (carbon monoxide) and fixing CO2 (carbon dioxide). We previously published the draft genome of this organism and recently submitted the complete genome sequence to GenBank.Results: The genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5 consists of a 3.74-Mb chromosome and a 133-kb megaplasmid that contains the genes responsible for utilization of carbon monoxide, carbon dioxide, and hydrogen. To our knowledge, this strain is the first one to be sequenced in the genus Oligotropha, the closest fully sequenced relatives being Bradyrhizobium sp. BTAi and USDA110 and Nitrobacter hamburgiensis X14. Analysis of the O. carboxidovorans genome reveals potential links between plasmid-encoded chemolithoautotrophy and chromosomally-encoded lipid metabolism. Comparative analysis of O. carboxidovorans with closely related species revealed differences in metabolic pathways, particularly in carbohydrate and lipid metabolism, as well as transport pathways.Conclusion: Oligotropha, Bradyrhizobium sp and Nitrobacter hamburgiensis X14 are phylogenetically proximal. Although there is significant conservation of genome organization between the species, there are major differences in many metabolic pathways that reflect the adaptive strategies unique to each species.

Original languageEnglish (US)
Article number511
JournalBMC Genomics
Volume11
Issue number1
DOIs
StatePublished - Sep 23 2010
Externally publishedYes

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Genome
Bacteria
Nitrobacter
Carbon Monoxide
Bradyrhizobium
Metabolic Networks and Pathways
Lipid Metabolism
Carbon Dioxide
Chemoautotrophic Growth
Nucleic Acid Databases
Carbohydrate Metabolism
Hydrogen
Plasmids
Chromosomes
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5. / Paul, Debarati; Bridges, Susan M.; Burgess, Shane C; Dandass, Yoginder S.; Lawrence, Mark L.

In: BMC Genomics, Vol. 11, No. 1, 511, 23.09.2010.

Research output: Contribution to journalArticle

Paul, Debarati ; Bridges, Susan M. ; Burgess, Shane C ; Dandass, Yoginder S. ; Lawrence, Mark L. / Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5. In: BMC Genomics. 2010 ; Vol. 11, No. 1.
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