Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination

Roberto Ramos-Sobrinho, Mayra M.M. Ferro, Tatsuya Nagata, Alina S. Puig, Cory Von Keith, Dahyana Santos Britto, Osman A. Gutierrez, Jean Philippe Marelli, Judith K. Brown

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

To analyze the DNA virome associated with cacao (Theobroma cacao L.) trees showing virus-like symptoms in Brazil (BR) and Puerto Rico (PR) during 2018-2019, total DNA was isolated from symptomatic leaves and subjected to high-throughput Illumina sequencing. The assembled complete badnaviral genome sequences were verified by PCR amplification, cloning, and DNA sequencing. Based on pairwise distances and phylogenetic analysis, three badnaviral genomes were identified, and these viruses were found to be isolates of the previously described cacao mild mosaic virus (CaMMV). The three genomes were 7,520, 7,524, and 7,514 bp in size for the isolates CaMMV-BR321, CaMMV-BR322, and CaMMV-PR3, respectively. Each genome contained four predicted open reading frames: ORFs 1-3 and ORFY. The CaMMV-PR3 isolate was identified as a probable recombinant, with a CaMMV-BR-like virus as the major parent.

Original languageEnglish (US)
Pages (from-to)2027-2031
Number of pages5
JournalArchives of virology
Volume166
Issue number7
DOIs
StatePublished - Jul 2021

ASJC Scopus subject areas

  • Virology

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