Complete recombinant silk-elastinlike protein-based tissue scaffold

Weiguo Qiu, Yiding Huang, Weibing Teng, Celine M. Cohn, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Due to their improved biocompatibility and specificity over synthetic materials, protein-based biomaterials, either derived from natural sources or genetically engineered, have been widely fabricated into nanofibrous scaffolds for tissue engineering applications. However, their inferior mechanical properties often require the reinforcement of protein-based tissue scaffolds using synthetic polymers. In this study, we report the electrospinning of a completely recombinant silk-elastinlike protein-based tissue scaffold with excellent mechanical properties and biocompatibility. In particular, SELP-47K containing tandemly repeated polypeptide sequences derived from native silk and elastin was electrospun into nanofibrous scaffolds, and stabilized via chemical vapor treatment and mechanical preconditioning. When fully hydrated in 1× - PBS at 37°C, mechanically preconditioned SELP-47K scaffolds displayed elastic moduli of 3.4-13.2 MPa, ultimate tensile strengths of 5.7-13.5 MPa, deformabilities of 100-130% strain, and resilience of 80.6-86.9%, closely matching or exceeding those of protein-synthetic blend polymeric scaffolds. Additionally, SELP-47K nanofibrous scaffolds promoted cell attachment and growth, demonstrating their in vitro biocompatibility.

Original languageEnglish (US)
Pages (from-to)3219-3227
Number of pages9
JournalBiomacromolecules
Volume11
Issue number12
DOIs
StatePublished - Dec 13 2010

Fingerprint

Tissue Scaffolds
Silk
Scaffolds
Scaffolds (biology)
Biocompatibility
Proteins
Elastin
Mechanical properties
Polypeptides
Electrospinning
Biocompatible Materials
Formability
Tissue engineering
Biomaterials
Reinforcement
Polymers
Tensile strength
Elastic moduli
Vapors
Peptides

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Qiu, W., Huang, Y., Teng, W., Cohn, C. M., Cappello, J., & Wu, X. (2010). Complete recombinant silk-elastinlike protein-based tissue scaffold. Biomacromolecules, 11(12), 3219-3227. https://doi.org/10.1021/bm100469w

Complete recombinant silk-elastinlike protein-based tissue scaffold. / Qiu, Weiguo; Huang, Yiding; Teng, Weibing; Cohn, Celine M.; Cappello, Joseph; Wu, Xiaoyi.

In: Biomacromolecules, Vol. 11, No. 12, 13.12.2010, p. 3219-3227.

Research output: Contribution to journalArticle

Qiu, W, Huang, Y, Teng, W, Cohn, CM, Cappello, J & Wu, X 2010, 'Complete recombinant silk-elastinlike protein-based tissue scaffold', Biomacromolecules, vol. 11, no. 12, pp. 3219-3227. https://doi.org/10.1021/bm100469w
Qiu, Weiguo ; Huang, Yiding ; Teng, Weibing ; Cohn, Celine M. ; Cappello, Joseph ; Wu, Xiaoyi. / Complete recombinant silk-elastinlike protein-based tissue scaffold. In: Biomacromolecules. 2010 ; Vol. 11, No. 12. pp. 3219-3227.
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