Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity

David W Montgomery, Charles F. Zukoski, Gul N. Shah, Arthur R. Buckley, Tadeusz Pacholczyk, Diane Haddock Russell

Research output: Contribution to journalArticle

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Abstract

Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)692-698
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number2
DOIs
StatePublished - Jun 15 1987

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Concanavalin A
Bioactivity
Prolactin
Lymphocytes
Lymphoma
Bioassay
Cell culture
Biological Assay
Rats
Immune Sera
Cultured Cells
Cell Count
Hormones
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity. / Montgomery, David W; Zukoski, Charles F.; Shah, Gul N.; Buckley, Arthur R.; Pacholczyk, Tadeusz; Russell, Diane Haddock.

In: Biochemical and Biophysical Research Communications, Vol. 145, No. 2, 15.06.1987, p. 692-698.

Research output: Contribution to journalArticle

Montgomery, David W ; Zukoski, Charles F. ; Shah, Gul N. ; Buckley, Arthur R. ; Pacholczyk, Tadeusz ; Russell, Diane Haddock. / Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 145, No. 2. pp. 692-698.
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