Abstract
Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 692-698 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 145 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 15 1987 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity. / Montgomery, David W; Zukoski, Charles F.; Shah, Gul N.; Buckley, Arthur R.; Pacholczyk, Tadeusz; Russell, Diane Haddock.
In: Biochemical and Biophysical Research Communications, Vol. 145, No. 2, 15.06.1987, p. 692-698.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity
AU - Montgomery, David W
AU - Zukoski, Charles F.
AU - Shah, Gul N.
AU - Buckley, Arthur R.
AU - Pacholczyk, Tadeusz
AU - Russell, Diane Haddock
PY - 1987/6/15
Y1 - 1987/6/15
N2 - Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.
AB - Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0023250734&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023250734&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)91020-5
DO - 10.1016/0006-291X(87)91020-5
M3 - Article
C2 - 3593364
AN - SCOPUS:0023250734
VL - 145
SP - 692
EP - 698
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -