Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity

David W. Montgomery; Charles F. Zukoski; Gul N. Shah; Arthur R. Buckley; Tadeusz Pacholczyk; Diane Haddock Russell

doi:10.1016/0006-291X(87)91020-5

Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity

David W. Montgomery, Charles F. Zukoski, Gul N. Shah, Arthur R. Buckley, Tadeusz Pacholczyk, Diane Haddock Russell

Nursing, College of

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb₂ node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb₂ lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.

Original language	English (US)
Pages (from-to)	692-698
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)91020-5
State	Published - Jun 15 1987

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity. / Montgomery, David W.; Zukoski, Charles F.; Shah, Gul N.; Buckley, Arthur R.; Pacholczyk, Tadeusz; Russell, Diane Haddock.

In: Biochemical and Biophysical Research Communications, Vol. 145, No. 2, 15.06.1987, p. 692-698.

Research output: Contribution to journal › Article › peer-review

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title = "Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity",

abstract = "Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.",

author = "Montgomery, {David W.} and Zukoski, {Charles F.} and Shah, {Gul N.} and Buckley, {Arthur R.} and Tadeusz Pacholczyk and Russell, {Diane Haddock}",

note = "Funding Information: We thank LaRue Mirr for typing and editorial assistance in paper. This work was supported in part by the Veterans the Arizona Kidney Foundation (CFZ) and USPHSG rant #ES-O3587 (DHR).",

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T1 - Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity

AU - Montgomery, David W.

AU - Zukoski, Charles F.

AU - Shah, Gul N.

AU - Buckley, Arthur R.

AU - Pacholczyk, Tadeusz

AU - Russell, Diane Haddock

N1 - Funding Information: We thank LaRue Mirr for typing and editorial assistance in paper. This work was supported in part by the Veterans the Arizona Kidney Foundation (CFZ) and USPHSG rant #ES-O3587 (DHR).

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N2 - Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.

AB - Culture supernanants from concanavalin A activated murine splenic mononuclear cells (splenocytes) were assayed for prolactin-like molecules using the Nb2 node lymphoma bioassay and immunostaining procedures. Con A stimulated splenocytes produced a factor with prolactin-like immunoreactivity that was mitogenic for Nb2 lymphoma cells. This factor first appeared in culture supernatants with the onset of lymphocyte mitogenesis and its activity was proportional to the number of cells cultured. In addition, splenocyte proliferation was inhibited by antiserum to rat prolactin, and this inhibition was partially reversed by added hormone. These findings show that murine splenocytes produce a substance with prolactin-like activity and suggest that this factor is essential for lymphocyte proliferation.

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