Concentration of bacteriophage lysates by filter chromatography

Sagar M. Goyal, Katherine S. Zerda, Charles P. Gerba

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

High-titered phage stocks are required for carrying out genetic and physicochemical studies on bacteriophages. This study describes a simple method for the concentration of coliphages MS-2 and T2 by charge-modified filters. Phage lysates were first clarified by filtration through serum-coated membrane filters. The clarified lysate was adjusted to pH 6 and passed through a Zeta-plus filter (30 S size). Greater than 99% virus adsorption occurred under these conditions. Adsorbed viruses were successfully eluted by small volumes of 3% beef extract (pH 10) which was subsequently neutralized with 3% beef extract (pH2). A concentration factor as great as 126 could be achieved by this method, with recoveries ranging from 54% to 97% (average = 69%).

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalJournal of Virological Methods
Volume1
Issue number2
DOIs
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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