Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater.

Charles P Gerba, S. R. Farrah, S. M. Goyal, C. Wallis, J. L. Melnick

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Methods are described for the efficient concentration of an enterovirus from large volumes of tap water, sewage, and seawater. Virus in acidified water (pH 3.5) in the presence of aluminum chloride was adsorbed to a 10-inch (ca. 25.4 cm) fiberglass depth cartridge and a 10-inch pleated epoxy-fiberglass filter in a series at flow rates of up to 37.8 liters (10 gallons) per min. Adsorbed viruses were eluted from the filters with glycine buffer (pH 10.5 to 11.5), and the eluate was reconcentrated by using a combination of aluminum flocculation followed by hydroextraction. With this procedure, poliovirus in large volumes of tap water, seawater, and sewage could be concentrated with an average efficiency of 52, 53, and 50%, respectively. It was demonstrated that this method is capable of detecting surface solid-associated viruses originating from sewage treatment plants. No difference in virus recovery between laboratory batch studies and a set-up with acid-salt injection was found. This unified scheme for the concentration of viruses has many advantages over previously described systems. These include: high operating flow rates, low weight and small size, effectiveness with a variety of waters with widely varying qualities, and filters with a high resistance to clogging.

Original languageEnglish (US)
Pages (from-to)540-548
Number of pages9
JournalApplied and Environmental Microbiology
Volume35
Issue number3
StatePublished - Mar 1978
Externally publishedYes

Fingerprint

Enterovirus
Seawater
tap water
Sewage
sewage
virus
seawater
Viruses
viruses
Water
fiberglass
filter
Flocculation
Satellite Viruses
water
aluminum
Poliovirus
aluminum chloride
Enterovirus C
Aluminum

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater. / Gerba, Charles P; Farrah, S. R.; Goyal, S. M.; Wallis, C.; Melnick, J. L.

In: Applied and Environmental Microbiology, Vol. 35, No. 3, 03.1978, p. 540-548.

Research output: Contribution to journalArticle

Gerba, Charles P ; Farrah, S. R. ; Goyal, S. M. ; Wallis, C. ; Melnick, J. L. / Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater. In: Applied and Environmental Microbiology. 1978 ; Vol. 35, No. 3. pp. 540-548.
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