Conditional knockout of the mouse NADPH-cytochrome P450 reductase gene

Lin Wu, Jun Gu, Yan Weng, Kerri Kluetzman, Pam Swiatek, Melissa Behr, Qing Yu Zhang, Xiaoliang Zhuo, Qiang Xie, Xinxin Ding

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

NADPH-cytochrome P450 reductase (CPR or POR) is the obligatory electron donor for all microsomal cytochrome P450 (CYP or P450)-catalyzed monooxygenase reactions. Disruption of the mouse Cpr gene has been reported to cause prenatal developmental defects and embryonic lethality. In this study, we generated a mouse model with a floxed Cpr allele (termed Cprlox). Homozygous Cprlox mice are fertile and without any histological abnormality or any change in CPR expression. The floxed Cpr allele was subsequently deleted efficiently by crossing Cprlox mice with transgenic mice having liver-specific Cre expression (Alb-Cre); the result was a decrease in the level of CPR protein in liver microsomes. The Cprlox strain will be valuable for conditional Cpr gene deletion and subsequent determination of the impact of CPR loss on the metabolism of endogenous and xenobiotic compounds, as well as on postnatal development and other biological functions.

Original languageEnglish (US)
Pages (from-to)177-181
Number of pages5
JournalGenesis
Volume36
Issue number4
DOIs
Publication statusPublished - Aug 1 2003
Externally publishedYes

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Keywords

  • Conditional knockout mice
  • Cre recombinase
  • Cytochrome P450
  • Liver
  • NADPH-cytochrome P450 reductase

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Wu, L., Gu, J., Weng, Y., Kluetzman, K., Swiatek, P., Behr, M., ... Ding, X. (2003). Conditional knockout of the mouse NADPH-cytochrome P450 reductase gene. Genesis, 36(4), 177-181. https://doi.org/10.1002/gene.10214