Confocal imaging with bilateral scanning and array detectors

G. J. Brakenhoff, K. Visscher

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

A novel arrangement for confocal microscopy is presented, in which the key elements are the use of an array detector such as a CCD for confocal image collection and the use of one double‐sided scanning mirror element for bilaterally scanning the object and collecting the data on the CCD. The resulting arrangement is shown to be capable of confocal imaging with high photon efficiency under adjustable conditions of confocality and varying image acquisition rates, i.e. from slow speed up to real‐time imaging. Either laser or conventional light sources may be utilized. In addition to CCD registration, direct observation by eye of the confocal image in fluorescence is also possible. 1992 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalJournal of Microscopy
Volume165
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

    Fingerprint

Keywords

  • Bilateral
  • CCD
  • array detector
  • confocal
  • fluorescence
  • laser
  • microscopy
  • real‐time
  • sectioning

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this