Confocal microscopy for the biological and material sciences: principle, applications, limitations

G. J. Brakenhoff, H. T. Voort, K. Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

In the confocal scanning light microscope a specific volume is sampled during the imaging process. The physical process is explained, together with how the size of the pinholes used affects the actual size of this volume. The thus produced 3-dimensional imaging is of high quality but subject to a number of limitations. A novel (bilateral scanning) arrangement is presented which may relieve some of these. Use is made in this approach of a double sided scanning mirror element and a charge coupled device (CCD) for image collection.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages121-127
Number of pages7
ISBN (Print)0819405299
StatePublished - Jan 1 1992
Externally publishedYes
EventInternational Conference on Scientific Optical Imaging - Georgetown, WA, USA
Duration: Nov 28 1990Dec 1 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1439
ISSN (Print)0277-786X

Other

OtherInternational Conference on Scientific Optical Imaging
CityGeorgetown, WA, USA
Period11/28/9012/1/90

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Brakenhoff, G. J., Voort, H. T., & Visscher, K. (1992). Confocal microscopy for the biological and material sciences: principle, applications, limitations. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 121-127). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1439). Publ by Int Soc for Optical Engineering.