Confocal microscopy for the life sciences

G. J. Brakenhoff, H. T. Voort, Koen Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the confocal scanning light microscope a specific volume is sampled during the imaging process. The physical process is explained, together with how the size of the pinholes used affect the actual size of this volume. The thus produced 3-D imaging is of high quality but subject to a number of limitations. A novel (bilateral scanning) arrangement is presented that may relieve some of these. In this approach, a double-sided scanning mirror element and a charge coupled device (CCD for image collection) are used.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsOliverio D. Soares, Annamaria V. Scheggi
PublisherPubl by Int Soc for Optical Engineering
Pages84-89
Number of pages6
ISBN (Print)081940652X
StatePublished - Jan 1 1992
Externally publishedYes
EventBioptics: Optics in Biomedicine and Environmental Sciences - Porto, Port
Duration: Mar 17 1991Mar 25 1991

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1524
ISSN (Print)0277-786X

Other

OtherBioptics: Optics in Biomedicine and Environmental Sciences
CityPorto, Port
Period3/17/913/25/91

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Brakenhoff, G. J., Voort, H. T., & Visscher, K. (1992). Confocal microscopy for the life sciences. In O. D. Soares, & A. V. Scheggi (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 84-89). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1524). Publ by Int Soc for Optical Engineering.