Confocal microscopy for the life sciences

G. J. Brakenhoff, H. T. Voort, Koen Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the confocal scanning light microscope a specific volume is sampled during the imaging process. The physical process is explained, together with how the size of the pinholes used affect the actual size of this volume. The thus produced 3-D imaging is of high quality but subject to a number of limitations. A novel (bilateral scanning) arrangement is presented that may relieve some of these. In this approach, a double-sided scanning mirror element and a charge coupled device (CCD for image collection) are used.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsOliverio D. Soares, Annamaria V. Scheggi
PublisherPubl by Int Soc for Optical Engineering
Pages84-89
Number of pages6
Volume1524
ISBN (Print)081940652X
StatePublished - 1992
Externally publishedYes
EventBioptics: Optics in Biomedicine and Environmental Sciences - Porto, Port
Duration: Mar 17 1991Mar 25 1991

Other

OtherBioptics: Optics in Biomedicine and Environmental Sciences
CityPorto, Port
Period3/17/913/25/91

Fingerprint

life sciences
Confocal microscopy
microscopy
Scanning
Charge coupled devices
scanning
charge coupled devices
Imaging techniques
pinholes
Density (specific gravity)
Mirrors
Microscopes
microscopes
mirrors

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Brakenhoff, G. J., Voort, H. T., & Visscher, K. (1992). Confocal microscopy for the life sciences. In O. D. Soares, & A. V. Scheggi (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1524, pp. 84-89). Publ by Int Soc for Optical Engineering.

Confocal microscopy for the life sciences. / Brakenhoff, G. J.; Voort, H. T.; Visscher, Koen.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Oliverio D. Soares; Annamaria V. Scheggi. Vol. 1524 Publ by Int Soc for Optical Engineering, 1992. p. 84-89.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Brakenhoff, GJ, Voort, HT & Visscher, K 1992, Confocal microscopy for the life sciences. in OD Soares & AV Scheggi (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1524, Publ by Int Soc for Optical Engineering, pp. 84-89, Bioptics: Optics in Biomedicine and Environmental Sciences, Porto, Port, 3/17/91.
Brakenhoff GJ, Voort HT, Visscher K. Confocal microscopy for the life sciences. In Soares OD, Scheggi AV, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1524. Publ by Int Soc for Optical Engineering. 1992. p. 84-89
Brakenhoff, G. J. ; Voort, H. T. ; Visscher, Koen. / Confocal microscopy for the life sciences. Proceedings of SPIE - The International Society for Optical Engineering. editor / Oliverio D. Soares ; Annamaria V. Scheggi. Vol. 1524 Publ by Int Soc for Optical Engineering, 1992. pp. 84-89
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