Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum

Michael L. Blumenfeld, Brandon S. Tackett, Laura K. Schirra, Jason M. Tyler, Oliver L A Monti Masel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have constructed an ultrahigh vacuum confocal fluorescence microscope with the purpose of performing single molecule spectroscopy under highly defined conditions. The microscope is designed for high stability while affording the capability of sample preparation, sample transfer, and optical detection in ultrahigh vacuum. It achieves near-diffraction-limited performance and allows the observation of single molecule fluorescence dynamics over extended periods of time. The design of the microscope is discussed in detail and the performance is demonstrated with single molecule fluorescence images and trajectories of N, N′ -dibutylperylene-3,4,9,10-dicarboxyimide deposited onto several different surfaces. This instrument further enhances the array of available surface science techniques, permitting spectroscopic investigations of molecule-surface interactions at the single molecule level and on insulating surfaces.

Original languageEnglish (US)
Article number103101
JournalReview of Scientific Instruments
Volume80
Issue number10
DOIs
StatePublished - 2009

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Fluorescence spectroscopy
Ultrahigh vacuum
ultrahigh vacuum
fluorescence
Molecules
Microscopes
spectroscopy
Fluorescence
microscopes
molecules
surface reactions
Diffraction
Trajectories
trajectories
Spectroscopy
preparation
diffraction

ASJC Scopus subject areas

  • Instrumentation

Cite this

Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum. / Blumenfeld, Michael L.; Tackett, Brandon S.; Schirra, Laura K.; Tyler, Jason M.; Monti Masel, Oliver L A.

In: Review of Scientific Instruments, Vol. 80, No. 10, 103101, 2009.

Research output: Contribution to journalArticle

Blumenfeld, Michael L. ; Tackett, Brandon S. ; Schirra, Laura K. ; Tyler, Jason M. ; Monti Masel, Oliver L A. / Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum. In: Review of Scientific Instruments. 2009 ; Vol. 80, No. 10.
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