Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum

Michael L. Blumenfeld, Brandon S. Tackett, Laura K. Schirra, Jason M. Tyler, Oliver L.A. Monti

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have constructed an ultrahigh vacuum confocal fluorescence microscope with the purpose of performing single molecule spectroscopy under highly defined conditions. The microscope is designed for high stability while affording the capability of sample preparation, sample transfer, and optical detection in ultrahigh vacuum. It achieves near-diffraction-limited performance and allows the observation of single molecule fluorescence dynamics over extended periods of time. The design of the microscope is discussed in detail and the performance is demonstrated with single molecule fluorescence images and trajectories of N, N′ -dibutylperylene-3,4,9,10-dicarboxyimide deposited onto several different surfaces. This instrument further enhances the array of available surface science techniques, permitting spectroscopic investigations of molecule-surface interactions at the single molecule level and on insulating surfaces.

Original languageEnglish (US)
Article number103101
JournalReview of Scientific Instruments
Volume80
Issue number10
DOIs
StatePublished - Nov 11 2009

ASJC Scopus subject areas

  • Instrumentation

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