Conformational Changes in a Soil Fulvic Acid Measured by Time-Dependent Fluorescence Depolarization

Charles H. Lochmüller, Steven S. Saavedra

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

The dependence of the aqueous conformation of a soil-derived fulvic acid on the independent parameters specifying the solution composition has been studied by time-dependent fluorescence depolarization spectroscopy. The molecular size and shape of the fluorescent components in fulvic acid were found to vary with solution pH, ionic strength, and sample concentration. These results indicate that this material behaves like a flexible polyelectrolyte in solutions approximating naturally occurring concentrations.

Original languageEnglish (US)
Pages (from-to)1978-1981
Number of pages4
JournalAnalytical chemistry
Volume58
Issue number9
DOIs
StatePublished - Aug 1986
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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