Congo red modulates ACh-induced Ca<sup>2+</sup> oscillations in single pancreatic acinar cells of mice

Ze Bing Huang, Hai Yan Wang, Na Na Sun, Jing Ke Wang, Meng Qin Zhao, Jian Xin Shen, Ming Gao, Ronald P Hammer, Xue Gong Fan, Jie Wu

Research output: Contribution to journalArticle

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Abstract

Aim: Congo red, a secondary diazo dye, is usually used as an indicator for the presence of amyloid fibrils. Recent studies show that congo red exerts neuroprotective effects in a variety of models of neurodegenerative diseases. However, its pharmacological profile remains unknown. In this study, we investigated the effects of congo red on ACh-induced Ca<sup>2+</sup> oscillations in mouse pancreatic acinar cells in vitro. Methods: Acutely dissociated pancreatic acinar cells of mice were prepared. A U-tube drug application system was used to deliver drugs into the bath. Intracellular Ca<sup>2+</sup> oscillations were monitored by whole-cell recording of Ca<sup>2+</sup> -activated Cl<sup>-</sup> currents and by using confocal Ca<sup>2+</sup> imaging. For intracellular drug application, the drug was added in pipette solution and diffused into cell after the whole-cell configuration was established. Results: Bath application of ACh (10 nmol/L) induced typical Ca<sup>2+</sup> oscillations in dissociated pancreatic acinar cells. Addition of congo red (1, 10, 100 μmol/L) dose-dependently enhanced Ach-induced Ca<sup>2+</sup> oscillations, but congo red alone did not induce any detectable response. Furthermore, this enhancement depended on the concentrations of ACh: congo red markedly enhanced the Ca<sup>2+</sup> oscillations induced by ACh (10-30 nmol/L), but did not alter the Ca<sup>2+</sup> oscillations induced by ACh (100-10000 nmol/L). Congo red also enhanced the Ca<sup>2+</sup> oscillations induced by bath application of IP<inf>3</inf> (30 μmol/L). Intracellular application of congo red failed to alter ACh-induced Ca<sup>2+</sup> oscillations. Conclusion: Congo red significantly modulates intracellular Ca<sup>2+</sup> signaling in pancreatic acinar cells, and this pharmacological effect should be fully considered when developing congo red as a novel therapeutic drug.

Original languageEnglish (US)
Pages (from-to)1514-1520
Number of pages7
JournalActa Pharmacologica Sinica
Volume35
Issue number12
DOIs
StatePublished - Nov 24 2014

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Congo Red
Acinar Cells
Baths
Pharmaceutical Preparations
Pharmacology
Neuroprotective Agents
Patch-Clamp Techniques
Amyloid
Neurodegenerative Diseases
Coloring Agents

Keywords

  • ACh; IP<inf>3</inf>; Ca<sup>2+</sup> oscillation; whole-cell recording; Ca<sup>2+</sup> image
  • Congo red
  • pancreatic acinar cell

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Huang, Z. B., Wang, H. Y., Sun, N. N., Wang, J. K., Zhao, M. Q., Shen, J. X., ... Wu, J. (2014). Congo red modulates ACh-induced Ca<sup>2+</sup> oscillations in single pancreatic acinar cells of mice. Acta Pharmacologica Sinica, 35(12), 1514-1520. https://doi.org/10.1038/aps.2014.94

Congo red modulates ACh-induced Ca<sup>2+</sup> oscillations in single pancreatic acinar cells of mice. / Huang, Ze Bing; Wang, Hai Yan; Sun, Na Na; Wang, Jing Ke; Zhao, Meng Qin; Shen, Jian Xin; Gao, Ming; Hammer, Ronald P; Fan, Xue Gong; Wu, Jie.

In: Acta Pharmacologica Sinica, Vol. 35, No. 12, 24.11.2014, p. 1514-1520.

Research output: Contribution to journalArticle

Huang, ZB, Wang, HY, Sun, NN, Wang, JK, Zhao, MQ, Shen, JX, Gao, M, Hammer, RP, Fan, XG & Wu, J 2014, 'Congo red modulates ACh-induced Ca<sup>2+</sup> oscillations in single pancreatic acinar cells of mice', Acta Pharmacologica Sinica, vol. 35, no. 12, pp. 1514-1520. https://doi.org/10.1038/aps.2014.94
Huang, Ze Bing ; Wang, Hai Yan ; Sun, Na Na ; Wang, Jing Ke ; Zhao, Meng Qin ; Shen, Jian Xin ; Gao, Ming ; Hammer, Ronald P ; Fan, Xue Gong ; Wu, Jie. / Congo red modulates ACh-induced Ca<sup>2+</sup> oscillations in single pancreatic acinar cells of mice. In: Acta Pharmacologica Sinica. 2014 ; Vol. 35, No. 12. pp. 1514-1520.
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abstract = "Aim: Congo red, a secondary diazo dye, is usually used as an indicator for the presence of amyloid fibrils. Recent studies show that congo red exerts neuroprotective effects in a variety of models of neurodegenerative diseases. However, its pharmacological profile remains unknown. In this study, we investigated the effects of congo red on ACh-induced Ca2+ oscillations in mouse pancreatic acinar cells in vitro. Methods: Acutely dissociated pancreatic acinar cells of mice were prepared. A U-tube drug application system was used to deliver drugs into the bath. Intracellular Ca2+ oscillations were monitored by whole-cell recording of Ca2+ -activated Cl- currents and by using confocal Ca2+ imaging. For intracellular drug application, the drug was added in pipette solution and diffused into cell after the whole-cell configuration was established. Results: Bath application of ACh (10 nmol/L) induced typical Ca2+ oscillations in dissociated pancreatic acinar cells. Addition of congo red (1, 10, 100 μmol/L) dose-dependently enhanced Ach-induced Ca2+ oscillations, but congo red alone did not induce any detectable response. Furthermore, this enhancement depended on the concentrations of ACh: congo red markedly enhanced the Ca2+ oscillations induced by ACh (10-30 nmol/L), but did not alter the Ca2+ oscillations induced by ACh (100-10000 nmol/L). Congo red also enhanced the Ca2+ oscillations induced by bath application of IP3 (30 μmol/L). Intracellular application of congo red failed to alter ACh-induced Ca2+ oscillations. Conclusion: Congo red significantly modulates intracellular Ca2+ signaling in pancreatic acinar cells, and this pharmacological effect should be fully considered when developing congo red as a novel therapeutic drug.",
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AU - Wang, Hai Yan

AU - Sun, Na Na

AU - Wang, Jing Ke

AU - Zhao, Meng Qin

AU - Shen, Jian Xin

AU - Gao, Ming

AU - Hammer, Ronald P

AU - Fan, Xue Gong

AU - Wu, Jie

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