Connexin 37 is dispensable for the control of the renin system and for positioning of renin-producing cells in the kidney

Charlotte Wagner, Lisa Kurtz, Frank Schweda, Alex Simon, Armin Kurtz

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Within the juxtaglomerular apparatus, reninproducing cells and endothelial cells of the afferent arterioles express connexin (Cx)37 and Cx40, which form abundant gap junctions among these cells. Deletion of Cx40 leads to strong hyperreninemia and ectopic localization of renin-producing cells; however, the relevance of Cx37 for the renin system in the kidney has not been investigated. We therefore studied renin expression and renin secretion in kidneys from Cx37deficient mice, both on normal salt diet and during chronic challenge of the renin system by pretreatment of mice with a low-salt diet in combination with an angiotensin I-converting enzyme inhibitor. This treatment procedure strongly enhances renin gene expression and renin secretion. We found that renal renin mRNA abundance and plasma renin concentration did not differ between wild-type and Cx37 -/- mice under normal conditions. The stimulation of renin gene expression and renin secretion by salt depletion was even more pronounced in CX37-/- as compared to wild-type mice. The regulation of renin secretion from isolated perfused kidneys by perfusion pressure and by angiotensin II was normal in CX37-/- mice. In addition, the localization of renin-expressing cells was also regular in CX37-/- kidneys. Finally, the expression pattern of other vascular Cxs such as Cx40, Cx43, and Cx45 was not altered in Cx37-/- kidneys. Our findings suggest that Cx37 is not essential for normal development and function of reninproducing cells. As a consequence, it appears unlikely that Cx40 exerts its important function in renin-producing cells via Cx37/Cx40 heteromeric gap junctions.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume459
Issue number1
DOIs
StatePublished - Nov 2009

Fingerprint

Renin
Kidney
Salts
Gap Junctions
Nutrition
connexin 37
Gene expression
Juxtaglomerular Apparatus
Sodium-Restricted Diet
Gene Expression
Connexin 43
Endothelial cells
Arterioles
Angiotensin-Converting Enzyme Inhibitors
Angiotensin II
Blood Vessels
Endothelial Cells
Perfusion

Keywords

  • Angiotensin
  • Gap junction
  • Kidney
  • Renal perfusion pressure
  • Renin

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)
  • Medicine(all)

Cite this

Connexin 37 is dispensable for the control of the renin system and for positioning of renin-producing cells in the kidney. / Wagner, Charlotte; Kurtz, Lisa; Schweda, Frank; Simon, Alex; Kurtz, Armin.

In: Pflugers Archiv European Journal of Physiology, Vol. 459, No. 1, 11.2009, p. 151-158.

Research output: Contribution to journalArticle

@article{2e47606520ab4545bbb151c1acc1cd95,
title = "Connexin 37 is dispensable for the control of the renin system and for positioning of renin-producing cells in the kidney",
abstract = "Within the juxtaglomerular apparatus, reninproducing cells and endothelial cells of the afferent arterioles express connexin (Cx)37 and Cx40, which form abundant gap junctions among these cells. Deletion of Cx40 leads to strong hyperreninemia and ectopic localization of renin-producing cells; however, the relevance of Cx37 for the renin system in the kidney has not been investigated. We therefore studied renin expression and renin secretion in kidneys from Cx37deficient mice, both on normal salt diet and during chronic challenge of the renin system by pretreatment of mice with a low-salt diet in combination with an angiotensin I-converting enzyme inhibitor. This treatment procedure strongly enhances renin gene expression and renin secretion. We found that renal renin mRNA abundance and plasma renin concentration did not differ between wild-type and Cx37 -/- mice under normal conditions. The stimulation of renin gene expression and renin secretion by salt depletion was even more pronounced in CX37-/- as compared to wild-type mice. The regulation of renin secretion from isolated perfused kidneys by perfusion pressure and by angiotensin II was normal in CX37-/- mice. In addition, the localization of renin-expressing cells was also regular in CX37-/- kidneys. Finally, the expression pattern of other vascular Cxs such as Cx40, Cx43, and Cx45 was not altered in Cx37-/- kidneys. Our findings suggest that Cx37 is not essential for normal development and function of reninproducing cells. As a consequence, it appears unlikely that Cx40 exerts its important function in renin-producing cells via Cx37/Cx40 heteromeric gap junctions.",
keywords = "Angiotensin, Gap junction, Kidney, Renal perfusion pressure, Renin",
author = "Charlotte Wagner and Lisa Kurtz and Frank Schweda and Alex Simon and Armin Kurtz",
year = "2009",
month = "11",
doi = "10.1007/s00424-009-0707-6",
language = "English (US)",
volume = "459",
pages = "151--158",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Connexin 37 is dispensable for the control of the renin system and for positioning of renin-producing cells in the kidney

AU - Wagner, Charlotte

AU - Kurtz, Lisa

AU - Schweda, Frank

AU - Simon, Alex

AU - Kurtz, Armin

PY - 2009/11

Y1 - 2009/11

N2 - Within the juxtaglomerular apparatus, reninproducing cells and endothelial cells of the afferent arterioles express connexin (Cx)37 and Cx40, which form abundant gap junctions among these cells. Deletion of Cx40 leads to strong hyperreninemia and ectopic localization of renin-producing cells; however, the relevance of Cx37 for the renin system in the kidney has not been investigated. We therefore studied renin expression and renin secretion in kidneys from Cx37deficient mice, both on normal salt diet and during chronic challenge of the renin system by pretreatment of mice with a low-salt diet in combination with an angiotensin I-converting enzyme inhibitor. This treatment procedure strongly enhances renin gene expression and renin secretion. We found that renal renin mRNA abundance and plasma renin concentration did not differ between wild-type and Cx37 -/- mice under normal conditions. The stimulation of renin gene expression and renin secretion by salt depletion was even more pronounced in CX37-/- as compared to wild-type mice. The regulation of renin secretion from isolated perfused kidneys by perfusion pressure and by angiotensin II was normal in CX37-/- mice. In addition, the localization of renin-expressing cells was also regular in CX37-/- kidneys. Finally, the expression pattern of other vascular Cxs such as Cx40, Cx43, and Cx45 was not altered in Cx37-/- kidneys. Our findings suggest that Cx37 is not essential for normal development and function of reninproducing cells. As a consequence, it appears unlikely that Cx40 exerts its important function in renin-producing cells via Cx37/Cx40 heteromeric gap junctions.

AB - Within the juxtaglomerular apparatus, reninproducing cells and endothelial cells of the afferent arterioles express connexin (Cx)37 and Cx40, which form abundant gap junctions among these cells. Deletion of Cx40 leads to strong hyperreninemia and ectopic localization of renin-producing cells; however, the relevance of Cx37 for the renin system in the kidney has not been investigated. We therefore studied renin expression and renin secretion in kidneys from Cx37deficient mice, both on normal salt diet and during chronic challenge of the renin system by pretreatment of mice with a low-salt diet in combination with an angiotensin I-converting enzyme inhibitor. This treatment procedure strongly enhances renin gene expression and renin secretion. We found that renal renin mRNA abundance and plasma renin concentration did not differ between wild-type and Cx37 -/- mice under normal conditions. The stimulation of renin gene expression and renin secretion by salt depletion was even more pronounced in CX37-/- as compared to wild-type mice. The regulation of renin secretion from isolated perfused kidneys by perfusion pressure and by angiotensin II was normal in CX37-/- mice. In addition, the localization of renin-expressing cells was also regular in CX37-/- kidneys. Finally, the expression pattern of other vascular Cxs such as Cx40, Cx43, and Cx45 was not altered in Cx37-/- kidneys. Our findings suggest that Cx37 is not essential for normal development and function of reninproducing cells. As a consequence, it appears unlikely that Cx40 exerts its important function in renin-producing cells via Cx37/Cx40 heteromeric gap junctions.

KW - Angiotensin

KW - Gap junction

KW - Kidney

KW - Renal perfusion pressure

KW - Renin

UR - http://www.scopus.com/inward/record.url?scp=73949113888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73949113888&partnerID=8YFLogxK

U2 - 10.1007/s00424-009-0707-6

DO - 10.1007/s00424-009-0707-6

M3 - Article

VL - 459

SP - 151

EP - 158

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 1

ER -