Conservation and purifying selection of transcribed genes located in a rice centromere

Chuanzhu Fan, Jason G. Walling, Jianwei - Zhang, Cory D. Hirsch, Jiming Jiang, Rod A Wing

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recombination is strongly suppressed in centromeric regions. In chromosomal regions with suppressed recombination, deleterious mutations can easily accumulate and cause degeneration of genes and genomes. Surprisingly, the centromere of chromosome8 (Cen8) of rice (Oryza sativa) contains several transcribed genes. However, it remains unclear as to what selective forces drive the evolution and existence of transcribed genes in Cen8. Sequencing of orthologous Cen8 regions from two additional Oryza species, Oryza glaberrima and Oryza brachyantha, which diverged from O. sativa 1 and 10 million years ago, respectively, revealed a set of seven transcribed Cen8 genes conserved across all three species. Chromatin immunoprecipitation analysis with the centromere-specific histone CENH3 confirmed that the sequenced orthologous regions are part of the functional centromere. All seven Cen8genes have undergone purifying selection, representing a striking phenomenon of active gene survival within a recombination-free zone over a long evolutionary time. The coding sequences of the Cen8 genes showed sequence divergence and mutation rates that were significantly reduced from those of genes located on the chromosome arms. This suggests that Oryza has a mechanism to maintain the fidelity and functionality of Cen8 genes, even when embedded in a sea of repetitive sequences and transposable elements.

Original languageEnglish (US)
Pages (from-to)2821-2830
Number of pages10
JournalPlant Cell
Volume23
Issue number8
DOIs
StatePublished - Aug 2011

Fingerprint

Centromere
centromeres
rice
Genes
genes
Genetic Recombination
Oryza
Oryza brachyantha
Oryza sativa
Oryza glaberrima
mutation
repetitive sequences
DNA Transposable Elements
Nucleic Acid Repetitive Sequences
Chromatin Immunoprecipitation
histones
transposons
Mutation Rate
chromatin
Histones

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Conservation and purifying selection of transcribed genes located in a rice centromere. / Fan, Chuanzhu; Walling, Jason G.; Zhang, Jianwei -; Hirsch, Cory D.; Jiang, Jiming; Wing, Rod A.

In: Plant Cell, Vol. 23, No. 8, 08.2011, p. 2821-2830.

Research output: Contribution to journalArticle

Fan, Chuanzhu ; Walling, Jason G. ; Zhang, Jianwei - ; Hirsch, Cory D. ; Jiang, Jiming ; Wing, Rod A. / Conservation and purifying selection of transcribed genes located in a rice centromere. In: Plant Cell. 2011 ; Vol. 23, No. 8. pp. 2821-2830.
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