Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A Wing

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalPlant Molecular Biology Reporter
Volume13
Issue number2
DOIs
StatePublished - Jun 1995
Externally publishedYes

Fingerprint

Bacterial Artificial Chromosomes
bacterial artificial chromosomes
Chromosomes
Arabidopsis
Libraries
Arabidopsis thaliana
Haploidy
haploidy
Clone Cells
Genes
clones
Genome Size
genome
Lycopersicon esculentum
Complementary DNA
tomatoes
Genome
genomics

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana. / Choi, Sangdun; Creelman, Robert A.; Mullet, John E.; Wing, Rod A.

In: Plant Molecular Biology Reporter, Vol. 13, No. 2, 06.1995, p. 124-128.

Research output: Contribution to journalArticle

Choi, Sangdun ; Creelman, Robert A. ; Mullet, John E. ; Wing, Rod A. / Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana. In: Plant Molecular Biology Reporter. 1995 ; Vol. 13, No. 2. pp. 124-128.
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