Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A Wing

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalPlant Molecular Biology Reporter
Volume13
Issue number2
DOIs
Publication statusPublished - Jun 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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