Context-specific regulation of LINE-1

Ivo Teneng, Vilius Stribinskis, Kenneth Ramos

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The present study was conducted to evaluate the contextual specificity of long interspersed nuclear element-1 (LINE-1 or L1) activation by cellular stress and the role of the aryl hydrocarbon receptor (AHR) transcription factor and oxidative stress in the gene activation response. Activation of the AHR by the genotoxic carcinogen benzo(a)pyrene (BaP) increased L1 expression in human cervical carcinoma (HeLa) cells, human microvascular endothelial cells (HMEC), mouse vascular smooth muscle cells (mVSMC) and mouse embryonic kidney cells (mK4). In contrast, challenge with a different AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or UV irradiation (10-20 J/ m2), induced L1 only in HeLa cells. Transactivation of the mouse L1Md-A5 promoter was observed in all cell types challenged with BaP, while TCDD was without effect, and UV only activated L1 in HeLa cells. Genetic and pharmacological experiments implicated the AHR and oxidative stress as contextual determinants of L1 inducibility by cellular stress.

Original languageEnglish (US)
Pages (from-to)1101-1110
Number of pages10
JournalGenes to Cells
Volume12
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

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Aryl Hydrocarbon Receptors
HeLa Cells
Transcriptional Activation
Oxidative Stress
Long Interspersed Nucleotide Elements
Benzo(a)pyrene
Vascular Smooth Muscle
Carcinogens
Smooth Muscle Myocytes
Transcription Factors
Endothelial Cells
Pharmacology
Ligands
Carcinoma
Kidney
Polychlorinated Dibenzodioxins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Context-specific regulation of LINE-1. / Teneng, Ivo; Stribinskis, Vilius; Ramos, Kenneth.

In: Genes to Cells, Vol. 12, No. 10, 10.2007, p. 1101-1110.

Research output: Contribution to journalArticle

Teneng, Ivo ; Stribinskis, Vilius ; Ramos, Kenneth. / Context-specific regulation of LINE-1. In: Genes to Cells. 2007 ; Vol. 12, No. 10. pp. 1101-1110.
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