Continuous-flow synthesis of alpha-gliadin peptides in an ultrasonic field and assay of their inhibition of intestinal sucrase activity.

Josef Vagner, P. Kocna, V. Krchnák

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Three dodecapeptide amides derived from the amino acid sequence of alpha-gliadin and four of their analogues were synthesized by continuous-flow solid-phase multiple peptide synthesis. Ultrasonic field conditions accelerated the coupling reaction without affecting purity. Biological tests of the synthetic fragments showed the relevance of toxicity prediction for preselection of immunogenic alpha-gliadin fragments.

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalPeptide Research
Volume4
Issue number5
StatePublished - Sep 1991
Externally publishedYes

Fingerprint

Sucrase
Gliadin
Ultrasonics
Assays
Peptides
Solid-Phase Synthesis Techniques
Amides
Toxicity
Amino Acid Sequence
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Continuous-flow synthesis of alpha-gliadin peptides in an ultrasonic field and assay of their inhibition of intestinal sucrase activity. / Vagner, Josef; Kocna, P.; Krchnák, V.

In: Peptide Research, Vol. 4, No. 5, 09.1991, p. 284-288.

Research output: Contribution to journalArticle

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