Continuous treatment of the insensitive munitions compound N-methyl-p-nitro aniline (MNA) in an upflow anaerobic sludge blanket (UASB) bioreactor

Christopher I. Olivares, Junqin Wang, Carlos D. Silva Luna, James A Field, Leif M Abrell, Maria Reye Sierra Alvarez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

N-methyl-p-nitroaniline (MNA) is an ingredient of insensitive munitions (IM) compounds that serves as a plasticizer and helps reduce unwanted detonations. As its use becomes widespread, MNA waste streams will be generated, necessitating viable treatment options. We studied MNA biodegradation and its inhibition potential to a representative anaerobic microbial population in wastewater treatment, methanogens. Anaerobic biodegradation and toxicity assays were performed and an up-flow anaerobic sludge blanket reactor (UASB) was operated to test continuous degradation of MNA. MNA was transformed almost stoichiometrically to N-methyl-p-phenylenediamine (MPD). MPD was not mineralized; however, it was readily autoxidized and polymerized extensively upon aeration at pH = 9. In the UASB reactor, MNA was fully degraded up to a loading rate of 297.5 μM MNA d-1. Regarding toxicity, MNA was very inhibitory to acetoclastic methanogens (IC50 = 103 μM) whereas MPD was much less toxic, causing only 13.9% inhibition at the highest concentration tested (1025 μM). The results taken as a whole indicate that anaerobic sludge can transform MNA to MPD continuously, and that the transformation decreases the cytotoxicity of the parent pollutant. MPD can be removed through extensive polymerization. These insights could help define efficient treatment options for waste streams polluted with MNA.

Original languageEnglish (US)
Pages (from-to)1116-1122
Number of pages7
JournalChemosphere
Volume144
DOIs
StatePublished - Feb 1 2016

Fingerprint

Bioreactors
Aniline
Sewage
bioreactor
sludge
biodegradation
toxicity
polymerization
Methanogens
aeration
transform
assay
Biodegradation
degradation
Toxicity
pollutant
aniline
Plasticizers
Poisons
Detonation

Keywords

  • Anaerobic
  • Biodegradation
  • Insensitive munitions
  • Microbial toxicity
  • N-methyl-p-nitroaniline
  • Nitroaromatic

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Continuous treatment of the insensitive munitions compound N-methyl-p-nitro aniline (MNA) in an upflow anaerobic sludge blanket (UASB) bioreactor. / Olivares, Christopher I.; Wang, Junqin; Silva Luna, Carlos D.; Field, James A; Abrell, Leif M; Sierra Alvarez, Maria Reye.

In: Chemosphere, Vol. 144, 01.02.2016, p. 1116-1122.

Research output: Contribution to journalArticle

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abstract = "N-methyl-p-nitroaniline (MNA) is an ingredient of insensitive munitions (IM) compounds that serves as a plasticizer and helps reduce unwanted detonations. As its use becomes widespread, MNA waste streams will be generated, necessitating viable treatment options. We studied MNA biodegradation and its inhibition potential to a representative anaerobic microbial population in wastewater treatment, methanogens. Anaerobic biodegradation and toxicity assays were performed and an up-flow anaerobic sludge blanket reactor (UASB) was operated to test continuous degradation of MNA. MNA was transformed almost stoichiometrically to N-methyl-p-phenylenediamine (MPD). MPD was not mineralized; however, it was readily autoxidized and polymerized extensively upon aeration at pH = 9. In the UASB reactor, MNA was fully degraded up to a loading rate of 297.5 μM MNA d-1. Regarding toxicity, MNA was very inhibitory to acetoclastic methanogens (IC50 = 103 μM) whereas MPD was much less toxic, causing only 13.9{\%} inhibition at the highest concentration tested (1025 μM). The results taken as a whole indicate that anaerobic sludge can transform MNA to MPD continuously, and that the transformation decreases the cytotoxicity of the parent pollutant. MPD can be removed through extensive polymerization. These insights could help define efficient treatment options for waste streams polluted with MNA.",
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