Contrast-enhanced photoacoustic imaging of live lobster nerve cord

Russell S Witte, S. Huang, S. Ashkenazi, K. Kim, M. O'Donnell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Photoacoustic imaging provides optical contrast with good penetration and high spatial resolution, making it an attractive tool for. noninvasive neural applications. We chose a commercial dye (NK2761) commonly used for optical imaging of membrane potential to enhance photoacoustic images of the live lobster nerve cord. The abdominal segment of the nerve cord was excised, stained and positioned in a custom neural recording system, enabling electrical stimulation and recording of compound action potentials. Photoacoustic and pulse echo images were also collected using a commercial ultrasound scanner and a 10-MHz linear probe. A wavelength-tunable pulsed laser source (Surelite™, 5 ns, ∼15 mJ, 30 mJ/cm2) operating at 20 Hz produced photoacoustic waves. Longitudinal photoacoustic scans of a 25-mm segment of the excised nerve cord, including ganglionic and axonal processes, were collected and displayed every 7 seconds. Without the contrast agent, an average of 10 scans produced a peak photoacoustic signal 6 dB over background noise. An additional 29 dB was obtained after the nerve was submerged in the dye for 20 minutes. The gain decreased to 23 dB and 14 dB at 810 nm and 910 nm, respectively - consistent with the dye's optical absorbance measured using a portable spectrometer. The contrast-enhanced photoacoustic signal had a broad spectrum peaking at 4 MHz, and, after high pass filtering, images approached 200-μm spatial resolution. The hybrid imaging system, which provided several hours of electrical stimulation and recording, represents a robust testbed to develop novel photoacoustic contrast for neural applications.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6437
DOIs
StatePublished - 2007
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - San Jose, CA, United States
Duration: Jan 21 2007Jan 24 2007

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
CountryUnited States
CitySan Jose, CA
Period1/21/071/24/07

Fingerprint

Photoacoustic effect
Imaging techniques
Dyes
Testbeds
Pulsed lasers
Acoustic noise
Imaging systems
Spectrometers
Ultrasonics
Membranes
Wavelength

Keywords

  • Contrast agent
  • Electrophysiology
  • Neural engineering
  • Optical imaging
  • Ultrasound
  • Voltage-sensitive dye

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Witte, R. S., Huang, S., Ashkenazi, S., Kim, K., & O'Donnell, M. (2007). Contrast-enhanced photoacoustic imaging of live lobster nerve cord. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6437). [64370J] https://doi.org/10.1117/12.701570

Contrast-enhanced photoacoustic imaging of live lobster nerve cord. / Witte, Russell S; Huang, S.; Ashkenazi, S.; Kim, K.; O'Donnell, M.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437 2007. 64370J.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Witte, RS, Huang, S, Ashkenazi, S, Kim, K & O'Donnell, M 2007, Contrast-enhanced photoacoustic imaging of live lobster nerve cord. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6437, 64370J, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.701570
Witte RS, Huang S, Ashkenazi S, Kim K, O'Donnell M. Contrast-enhanced photoacoustic imaging of live lobster nerve cord. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437. 2007. 64370J https://doi.org/10.1117/12.701570
Witte, Russell S ; Huang, S. ; Ashkenazi, S. ; Kim, K. ; O'Donnell, M. / Contrast-enhanced photoacoustic imaging of live lobster nerve cord. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437 2007.
@inproceedings{23255259e30842b1922dbb4098377b56,
title = "Contrast-enhanced photoacoustic imaging of live lobster nerve cord",
abstract = "Photoacoustic imaging provides optical contrast with good penetration and high spatial resolution, making it an attractive tool for. noninvasive neural applications. We chose a commercial dye (NK2761) commonly used for optical imaging of membrane potential to enhance photoacoustic images of the live lobster nerve cord. The abdominal segment of the nerve cord was excised, stained and positioned in a custom neural recording system, enabling electrical stimulation and recording of compound action potentials. Photoacoustic and pulse echo images were also collected using a commercial ultrasound scanner and a 10-MHz linear probe. A wavelength-tunable pulsed laser source (Surelite™, 5 ns, ∼15 mJ, 30 mJ/cm2) operating at 20 Hz produced photoacoustic waves. Longitudinal photoacoustic scans of a 25-mm segment of the excised nerve cord, including ganglionic and axonal processes, were collected and displayed every 7 seconds. Without the contrast agent, an average of 10 scans produced a peak photoacoustic signal 6 dB over background noise. An additional 29 dB was obtained after the nerve was submerged in the dye for 20 minutes. The gain decreased to 23 dB and 14 dB at 810 nm and 910 nm, respectively - consistent with the dye's optical absorbance measured using a portable spectrometer. The contrast-enhanced photoacoustic signal had a broad spectrum peaking at 4 MHz, and, after high pass filtering, images approached 200-μm spatial resolution. The hybrid imaging system, which provided several hours of electrical stimulation and recording, represents a robust testbed to develop novel photoacoustic contrast for neural applications.",
keywords = "Contrast agent, Electrophysiology, Neural engineering, Optical imaging, Ultrasound, Voltage-sensitive dye",
author = "Witte, {Russell S} and S. Huang and S. Ashkenazi and K. Kim and M. O'Donnell",
year = "2007",
doi = "10.1117/12.701570",
language = "English (US)",
isbn = "081946550X",
volume = "6437",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Contrast-enhanced photoacoustic imaging of live lobster nerve cord

AU - Witte, Russell S

AU - Huang, S.

AU - Ashkenazi, S.

AU - Kim, K.

AU - O'Donnell, M.

PY - 2007

Y1 - 2007

N2 - Photoacoustic imaging provides optical contrast with good penetration and high spatial resolution, making it an attractive tool for. noninvasive neural applications. We chose a commercial dye (NK2761) commonly used for optical imaging of membrane potential to enhance photoacoustic images of the live lobster nerve cord. The abdominal segment of the nerve cord was excised, stained and positioned in a custom neural recording system, enabling electrical stimulation and recording of compound action potentials. Photoacoustic and pulse echo images were also collected using a commercial ultrasound scanner and a 10-MHz linear probe. A wavelength-tunable pulsed laser source (Surelite™, 5 ns, ∼15 mJ, 30 mJ/cm2) operating at 20 Hz produced photoacoustic waves. Longitudinal photoacoustic scans of a 25-mm segment of the excised nerve cord, including ganglionic and axonal processes, were collected and displayed every 7 seconds. Without the contrast agent, an average of 10 scans produced a peak photoacoustic signal 6 dB over background noise. An additional 29 dB was obtained after the nerve was submerged in the dye for 20 minutes. The gain decreased to 23 dB and 14 dB at 810 nm and 910 nm, respectively - consistent with the dye's optical absorbance measured using a portable spectrometer. The contrast-enhanced photoacoustic signal had a broad spectrum peaking at 4 MHz, and, after high pass filtering, images approached 200-μm spatial resolution. The hybrid imaging system, which provided several hours of electrical stimulation and recording, represents a robust testbed to develop novel photoacoustic contrast for neural applications.

AB - Photoacoustic imaging provides optical contrast with good penetration and high spatial resolution, making it an attractive tool for. noninvasive neural applications. We chose a commercial dye (NK2761) commonly used for optical imaging of membrane potential to enhance photoacoustic images of the live lobster nerve cord. The abdominal segment of the nerve cord was excised, stained and positioned in a custom neural recording system, enabling electrical stimulation and recording of compound action potentials. Photoacoustic and pulse echo images were also collected using a commercial ultrasound scanner and a 10-MHz linear probe. A wavelength-tunable pulsed laser source (Surelite™, 5 ns, ∼15 mJ, 30 mJ/cm2) operating at 20 Hz produced photoacoustic waves. Longitudinal photoacoustic scans of a 25-mm segment of the excised nerve cord, including ganglionic and axonal processes, were collected and displayed every 7 seconds. Without the contrast agent, an average of 10 scans produced a peak photoacoustic signal 6 dB over background noise. An additional 29 dB was obtained after the nerve was submerged in the dye for 20 minutes. The gain decreased to 23 dB and 14 dB at 810 nm and 910 nm, respectively - consistent with the dye's optical absorbance measured using a portable spectrometer. The contrast-enhanced photoacoustic signal had a broad spectrum peaking at 4 MHz, and, after high pass filtering, images approached 200-μm spatial resolution. The hybrid imaging system, which provided several hours of electrical stimulation and recording, represents a robust testbed to develop novel photoacoustic contrast for neural applications.

KW - Contrast agent

KW - Electrophysiology

KW - Neural engineering

KW - Optical imaging

KW - Ultrasound

KW - Voltage-sensitive dye

UR - http://www.scopus.com/inward/record.url?scp=34247329558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247329558&partnerID=8YFLogxK

U2 - 10.1117/12.701570

DO - 10.1117/12.701570

M3 - Conference contribution

AN - SCOPUS:34247329558

SN - 081946550X

SN - 9780819465504

VL - 6437

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -