Contrast-free microscopic assessment of glioblastoma bioposy specimens prior to biobanking

Joseph Georges, Aqib Zehri, Elizabeth Carlson, Joshua Nichols, Michael A. Mooney, Nikolay L. Martirosyan, Layla Ghaffari, M. Yashar S. Kalani, Jennifer Eschbacher, Burt Feuerstein, Trent Anderson, Mark C. Preul, Kendall Van Keuren-Jensen, Peter Nakaji

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Glioblastoma is the most common primary brain tumor with a median 12- to 15-month patient survival. Improving patient survival involves better understanding the biological mechanisms of glioblastoma tumorigenesis and seeking targeted molecular therapies. Central to furthering these advances is the collection and storage of surgical biopsies (biobanking) for research. This paper addresses an imaging modality, confocal reflectance microscopy (CRM), for safely screening glioblastoma biopsy samples prior to biobanking to increase the quality of tissue provided for research and clinical trials. These data indicate that CRM can immediately identify cellularity of tissue biopsies from animal models of glioblastoma. When screening fresh human biopsy samples, CRM can differentiate a cellular glioblastoma biopsy from a necrotic biopsy without altering DNA, RNA, or protein expression of sampled tissue. These data illustrate CRM's potential for rapidly and safely screening clinical biopsy samples prior to biobanking, which demonstrates its potential as an effective screening technique that can improve the quality of tissue biobanked for patients with glioblastoma.

Original languageEnglish (US)
Article numberE8
JournalNeurosurgical focus
Volume36
Issue number2
DOIs
StatePublished - Feb 26 2014

Keywords

  • Biobank
  • Biopsy
  • Confocal reflectance microscopy
  • Glioblastoma

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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