Control of diuresis in the yellow fever mosquito Aedes aegypti: Evidence for similar mechanisms in the male and female

L. Plawner, Thomas L Pannabecker, S. Laufer, M. D. Baustian, K. W. Beyenbach

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study was undertaken to determine whether males of the mosquito Aedes aegypti have diuretic mechanisms similar to the female, even though males do not take blood meals and, therefore would not seem to require mechanisms for rapid diuresis. In particular, the effects of secretagogues extracted from female and male mosquito heads on the Malpighian tubules of both sexes were tested. Male Malpighian tubules are considerably smaller than female tubules and they secrete Na, Cl and water in vitro at 1 6 the rates of female tubules. However, the concentrations of Na, K and Cl are similar in fluid secreted by female and male tubules. The female head extract significantly increases Na, Cl and water secretion in tubules of both sexes. Likewise, the male head extract significantly increases Na, Cl and water secretion in tubules of both sexes. K secretion by female tubules increases after treatment with female extract but decreases after treatment with male extract. K secretion by male tubules is not affected by female or male extracts. Female and male head extracts also share the same effects on transepithelial voltage and resistance across isolated perfused Malpighian tubules. Cyclic AMP, a known stimulator of Na, Cl and water secretion in female Malpighian tubules also significantly increases Na, Cl and water secretion in male tubules. Therefore, Malpighian tubules of the male and female mosquitoes appear to have similar ion transport mechanisms for NaCl and fluid secretion as well as similar diuretic hormones and receptors. Only quantitative differences exist between secretion of Na, Cl and water in the male and female mosquito, reflecting the larger NaCl and fluid loads consumed by the female.

Original languageEnglish (US)
JournalJournal of Insect Physiology
Volume37
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Yellow Fever
diuresis
Aedes
Diuresis
Aedes aegypti
Culicidae
Malpighian Tubules
Malpighian tubules
secretion
Water
extracts
Head
water
Diuretics
gender
diuretic hormone
Fluids and Secretions
diuretics
Ion Transport
blood meal

Keywords

  • Aedes aegypti
  • cAMP
  • diuretic mechanisms
  • head extract with diuretic activity
  • Malpighian tubules
  • Na, K and Cl secretion

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Control of diuresis in the yellow fever mosquito Aedes aegypti : Evidence for similar mechanisms in the male and female. / Plawner, L.; Pannabecker, Thomas L; Laufer, S.; Baustian, M. D.; Beyenbach, K. W.

In: Journal of Insect Physiology, Vol. 37, No. 2, 1991.

Research output: Contribution to journalArticle

@article{177384b52ed84bb39602c4c9b1398f7c,
title = "Control of diuresis in the yellow fever mosquito Aedes aegypti: Evidence for similar mechanisms in the male and female",
abstract = "This study was undertaken to determine whether males of the mosquito Aedes aegypti have diuretic mechanisms similar to the female, even though males do not take blood meals and, therefore would not seem to require mechanisms for rapid diuresis. In particular, the effects of secretagogues extracted from female and male mosquito heads on the Malpighian tubules of both sexes were tested. Male Malpighian tubules are considerably smaller than female tubules and they secrete Na, Cl and water in vitro at 1 6 the rates of female tubules. However, the concentrations of Na, K and Cl are similar in fluid secreted by female and male tubules. The female head extract significantly increases Na, Cl and water secretion in tubules of both sexes. Likewise, the male head extract significantly increases Na, Cl and water secretion in tubules of both sexes. K secretion by female tubules increases after treatment with female extract but decreases after treatment with male extract. K secretion by male tubules is not affected by female or male extracts. Female and male head extracts also share the same effects on transepithelial voltage and resistance across isolated perfused Malpighian tubules. Cyclic AMP, a known stimulator of Na, Cl and water secretion in female Malpighian tubules also significantly increases Na, Cl and water secretion in male tubules. Therefore, Malpighian tubules of the male and female mosquitoes appear to have similar ion transport mechanisms for NaCl and fluid secretion as well as similar diuretic hormones and receptors. Only quantitative differences exist between secretion of Na, Cl and water in the male and female mosquito, reflecting the larger NaCl and fluid loads consumed by the female.",
keywords = "Aedes aegypti, cAMP, diuretic mechanisms, head extract with diuretic activity, Malpighian tubules, Na, K and Cl secretion",
author = "L. Plawner and Pannabecker, {Thomas L} and S. Laufer and Baustian, {M. D.} and Beyenbach, {K. W.}",
year = "1991",
doi = "10.1016/0022-1910(91)90097-J",
language = "English (US)",
volume = "37",
journal = "Journal of Insect Physiology",
issn = "0022-1910",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Control of diuresis in the yellow fever mosquito Aedes aegypti

T2 - Evidence for similar mechanisms in the male and female

AU - Plawner, L.

AU - Pannabecker, Thomas L

AU - Laufer, S.

AU - Baustian, M. D.

AU - Beyenbach, K. W.

PY - 1991

Y1 - 1991

N2 - This study was undertaken to determine whether males of the mosquito Aedes aegypti have diuretic mechanisms similar to the female, even though males do not take blood meals and, therefore would not seem to require mechanisms for rapid diuresis. In particular, the effects of secretagogues extracted from female and male mosquito heads on the Malpighian tubules of both sexes were tested. Male Malpighian tubules are considerably smaller than female tubules and they secrete Na, Cl and water in vitro at 1 6 the rates of female tubules. However, the concentrations of Na, K and Cl are similar in fluid secreted by female and male tubules. The female head extract significantly increases Na, Cl and water secretion in tubules of both sexes. Likewise, the male head extract significantly increases Na, Cl and water secretion in tubules of both sexes. K secretion by female tubules increases after treatment with female extract but decreases after treatment with male extract. K secretion by male tubules is not affected by female or male extracts. Female and male head extracts also share the same effects on transepithelial voltage and resistance across isolated perfused Malpighian tubules. Cyclic AMP, a known stimulator of Na, Cl and water secretion in female Malpighian tubules also significantly increases Na, Cl and water secretion in male tubules. Therefore, Malpighian tubules of the male and female mosquitoes appear to have similar ion transport mechanisms for NaCl and fluid secretion as well as similar diuretic hormones and receptors. Only quantitative differences exist between secretion of Na, Cl and water in the male and female mosquito, reflecting the larger NaCl and fluid loads consumed by the female.

AB - This study was undertaken to determine whether males of the mosquito Aedes aegypti have diuretic mechanisms similar to the female, even though males do not take blood meals and, therefore would not seem to require mechanisms for rapid diuresis. In particular, the effects of secretagogues extracted from female and male mosquito heads on the Malpighian tubules of both sexes were tested. Male Malpighian tubules are considerably smaller than female tubules and they secrete Na, Cl and water in vitro at 1 6 the rates of female tubules. However, the concentrations of Na, K and Cl are similar in fluid secreted by female and male tubules. The female head extract significantly increases Na, Cl and water secretion in tubules of both sexes. Likewise, the male head extract significantly increases Na, Cl and water secretion in tubules of both sexes. K secretion by female tubules increases after treatment with female extract but decreases after treatment with male extract. K secretion by male tubules is not affected by female or male extracts. Female and male head extracts also share the same effects on transepithelial voltage and resistance across isolated perfused Malpighian tubules. Cyclic AMP, a known stimulator of Na, Cl and water secretion in female Malpighian tubules also significantly increases Na, Cl and water secretion in male tubules. Therefore, Malpighian tubules of the male and female mosquitoes appear to have similar ion transport mechanisms for NaCl and fluid secretion as well as similar diuretic hormones and receptors. Only quantitative differences exist between secretion of Na, Cl and water in the male and female mosquito, reflecting the larger NaCl and fluid loads consumed by the female.

KW - Aedes aegypti

KW - cAMP

KW - diuretic mechanisms

KW - head extract with diuretic activity

KW - Malpighian tubules

KW - Na, K and Cl secretion

UR - http://www.scopus.com/inward/record.url?scp=0000573891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000573891&partnerID=8YFLogxK

U2 - 10.1016/0022-1910(91)90097-J

DO - 10.1016/0022-1910(91)90097-J

M3 - Article

AN - SCOPUS:0000573891

VL - 37

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

SN - 0022-1910

IS - 2

ER -