Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis

Tomohiro Kiyosue, Nir Ohad, Ramin Yadegari, Mike Hannon, Jose Dinneny, Derek Wells, Anat Katz, Linda Margossian, John J. Harada, Robert B. Goldberg, Robert L. Fischer

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. and Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.

Original languageEnglish (US)
Pages (from-to)4186-4191
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number7
DOIs
StatePublished - Mar 30 1999
Externally publishedYes

Fingerprint

Endosperm
Arabidopsis
Fertilization
Embryonic Development
Spermatozoa
Genes
Reproduction
Ovum
Plant Proteins
Haploidy
Cell Nucleus
Embryonic Structures
Alleles
Mothers
Cell Proliferation
Mutation
Proteins

Keywords

  • Apomixis
  • Embryo
  • Reproduction
  • Seed

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis. / Kiyosue, Tomohiro; Ohad, Nir; Yadegari, Ramin; Hannon, Mike; Dinneny, Jose; Wells, Derek; Katz, Anat; Margossian, Linda; Harada, John J.; Goldberg, Robert B.; Fischer, Robert L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 7, 30.03.1999, p. 4186-4191.

Research output: Contribution to journalArticle

Kiyosue, T, Ohad, N, Yadegari, R, Hannon, M, Dinneny, J, Wells, D, Katz, A, Margossian, L, Harada, JJ, Goldberg, RB & Fischer, RL 1999, 'Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis', Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 7, pp. 4186-4191. https://doi.org/10.1073/pnas.96.7.4186
Kiyosue, Tomohiro ; Ohad, Nir ; Yadegari, Ramin ; Hannon, Mike ; Dinneny, Jose ; Wells, Derek ; Katz, Anat ; Margossian, Linda ; Harada, John J. ; Goldberg, Robert B. ; Fischer, Robert L. / Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 7. pp. 4186-4191.
@article{7d9145ba303f43cabe4e9e7d2705ac9d,
title = "Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis",
abstract = "Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. and Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.",
keywords = "Apomixis, Embryo, Reproduction, Seed",
author = "Tomohiro Kiyosue and Nir Ohad and Ramin Yadegari and Mike Hannon and Jose Dinneny and Derek Wells and Anat Katz and Linda Margossian and Harada, {John J.} and Goldberg, {Robert B.} and Fischer, {Robert L.}",
year = "1999",
month = "3",
day = "30",
doi = "10.1073/pnas.96.7.4186",
language = "English (US)",
volume = "96",
pages = "4186--4191",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis

AU - Kiyosue, Tomohiro

AU - Ohad, Nir

AU - Yadegari, Ramin

AU - Hannon, Mike

AU - Dinneny, Jose

AU - Wells, Derek

AU - Katz, Anat

AU - Margossian, Linda

AU - Harada, John J.

AU - Goldberg, Robert B.

AU - Fischer, Robert L.

PY - 1999/3/30

Y1 - 1999/3/30

N2 - Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. and Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.

AB - Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. and Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.

KW - Apomixis

KW - Embryo

KW - Reproduction

KW - Seed

UR - http://www.scopus.com/inward/record.url?scp=0344980397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344980397&partnerID=8YFLogxK

U2 - 10.1073/pnas.96.7.4186

DO - 10.1073/pnas.96.7.4186

M3 - Article

C2 - 10097185

AN - SCOPUS:0344980397

VL - 96

SP - 4186

EP - 4191

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -