Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA

B. Leticia Rodriguez, Xinran Li, Kaoru Kiguchi, John Digiovanni, Evan C. Unger, Zhengrong Cui

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Aim: Previously, it was shown that treatment of tumor-bearing mice with an RNA replicase-based plasmid that produces dsRNA when transfected into tumor cells significantly inhibited the tumor growth. In the present study, the feasibility of further improving the anti-tumor activity of the RNA replicase-based plasmid by targeting it into tumors cells was evaluated. Material & methods: An EGF-conjugated, polyethylene glycosylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-β, into EGF receptor-overexpressing human breast cancer cells (MDA-MB-468) in vitro and in vivo. Results: Delivery of pSIN-β using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-β carried by the EGF receptor-targeted liposome caused the complete regression of MDA-MB-468 tumors in mice, probably due to the enhancement of its proapoptotic, antiproliferative and antiangiogenic activities. Discussion: Tumor-targeted RNA replicase-based plasmids hold a strong potential in tumor therapy.

Original languageEnglish (US)
Pages (from-to)475-491
Number of pages17
JournalNanomedicine
Volume7
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • apoptosis
  • breast tumor
  • dsRNA therapy
  • epidermoid carcinoma
  • liposome
  • plasmid uptake

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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