Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA

B. Leticia Rodriguez, Xinran Li, Kaoru Kiguchi, John Digiovanni, Evan C Unger, Zhengrong Cui

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: Previously, it was shown that treatment of tumor-bearing mice with an RNA replicase-based plasmid that produces dsRNA when transfected into tumor cells significantly inhibited the tumor growth. In the present study, the feasibility of further improving the anti-tumor activity of the RNA replicase-based plasmid by targeting it into tumors cells was evaluated. Material & methods: An EGF-conjugated, polyethylene glycosylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-β, into EGF receptor-overexpressing human breast cancer cells (MDA-MB-468) in vitro and in vivo. Results: Delivery of pSIN-β using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-β carried by the EGF receptor-targeted liposome caused the complete regression of MDA-MB-468 tumors in mice, probably due to the enhancement of its proapoptotic, antiproliferative and antiangiogenic activities. Discussion: Tumor-targeted RNA replicase-based plasmids hold a strong potential in tumor therapy.

Original languageEnglish (US)
Pages (from-to)475-491
Number of pages17
JournalNanomedicine
Volume7
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

RNA Replicase
RNA
plasmid
Epidermal Growth Factor Receptor
tumor
Tumors
Plasmids
DNA
Growth
Liposomes
Neoplasms
cancer
regression
Cells
Bearings (structural)
Epidermal Growth Factor
Polyethylene
targeting
Polyethylenes
Squamous Cell Carcinoma

Keywords

  • apoptosis
  • breast tumor
  • dsRNA therapy
  • epidermoid carcinoma
  • liposome
  • plasmid uptake

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA. / Rodriguez, B. Leticia; Li, Xinran; Kiguchi, Kaoru; Digiovanni, John; Unger, Evan C; Cui, Zhengrong.

In: Nanomedicine, Vol. 7, No. 4, 04.2012, p. 475-491.

Research output: Contribution to journalArticle

Rodriguez, B. Leticia ; Li, Xinran ; Kiguchi, Kaoru ; Digiovanni, John ; Unger, Evan C ; Cui, Zhengrong. / Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA. In: Nanomedicine. 2012 ; Vol. 7, No. 4. pp. 475-491.
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