Convenient and efficient synthesis of a lanthanide 3+ - Coordinated, diethylene triamine pentaacetic acid labeled biopolymer as an assay for the cholecystokinin B receptor

F. Gao, H. Handl, J. Vagner, V. Hruby, R. Gillies

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To develop an assay for the cholecystokinin B receptor with an Eu 3+-labeled cholecystokinin peptide via a diethylene triamine pentaacetic acid chelating linker, a commercial dianhydride diethylene triamine pentaacetic acid precursor was directly attached to the N-terminus of cholecystokinin peptides by a solid-phase synthesis method with a satisfactory yield and purity after reverse-phase high-performance liquid chromatography separation. Lanthanide was then coordinated to the peptide via a diethylene triamine pentaacetic acid bifunctional agent. This method is a useful approach to the large-scale synthesis of lanthanide 3+-coordinated, diethylene triamine pentaacetic acid labeled biopolymers. This research provides not only a simple and convenient method for the preparation of lanthanide-based peptide ligand libraries but also possible lanthanide-based highthroughput screening of peptide receptors with a timeresolved fluorescence assay system. Five biopolymers were synthesized and characterized with high-resolution electrospray ionization in this study.

Original languageEnglish (US)
Pages (from-to)2683-2688
Number of pages6
JournalJournal of Applied Polymer Science
Volume106
Issue number4
DOIs
StatePublished - Nov 15 2007

Keywords

  • Biopolymers
  • Fluorescence
  • High performance liquid chromatography (HPLC)
  • Peptides
  • Synthesis

ASJC Scopus subject areas

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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