Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD)

Scott D Lick, S. K. Alpard, J. B. Zwischenberger, R. L. Brunston, A. Haque, A. Bidani

Research output: Contribution to journalArticle

Abstract

Purpose: The shortage of lung transplant donors may be lessened by the use of non-heart-beating lung donors (NBHLD). Optimal minute ventilation of the NBHLD is not known. We hypothesized that a moderately high minute ventilation relative to tissue metabolism to cause alveolar-induced respiratory alkalosis will ameliorate lung tissue acidosis and improve lung parenchyma viability. Methods: After sacrifice, Sprague-Dawley rats were separated into 4 groups of 8 rats each: deflated, non-ventilated controls (group I); 2 breaths/min (group II); 10 breaths/min (group III); and 20 breaths/min (group IV). All ventilation was done with room air and volumetrically set for full chest wall expansion. Two hours after sacrifice, the lungs were excised and flushed with tryphan blue vital dye to assess cell viability. Percent non-viable type II pneumocytes/10 HPF were counted in a blinded manner by a single pathologist. Results: GROUP % non-viable cells p value I (control) 37.6 ± 5.7 (I vs. II) p = 0.195 II (2 breath/min) 25.0 ± 3.7 (II vs. III) p = 0.002 III (10 breath/min) 7.7 ± 1.5 (I vs. III) p < 0.001 IV (20 breaths/min) 42.5 ± 4.3 (II vs. IV) p < 0.05 (III vs. IV) p < 0.001 Conclusions: Ventilation of the NBHLD at a moderately high rate (10 breath/min) relative to lung metabolism gives significantly improved cell viability over low frequency or high-frequency ventilation.

Original languageEnglish (US)
JournalChest
Volume114
Issue number4 SUPPL.
StatePublished - Oct 1998
Externally publishedYes

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High-Frequency Ventilation
Ventilation
Tissue Donors
Lung
Cell Survival
Respiratory Alkalosis
Alveolar Epithelial Cells
Thoracic Wall
Acidosis
Sprague Dawley Rats
Coloring Agents
Air
Control Groups

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Lick, S. D., Alpard, S. K., Zwischenberger, J. B., Brunston, R. L., Haque, A., & Bidani, A. (1998). Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD). Chest, 114(4 SUPPL.).

Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD). / Lick, Scott D; Alpard, S. K.; Zwischenberger, J. B.; Brunston, R. L.; Haque, A.; Bidani, A.

In: Chest, Vol. 114, No. 4 SUPPL., 10.1998.

Research output: Contribution to journalArticle

Lick, SD, Alpard, SK, Zwischenberger, JB, Brunston, RL, Haque, A & Bidani, A 1998, 'Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD)', Chest, vol. 114, no. 4 SUPPL..
Lick SD, Alpard SK, Zwischenberger JB, Brunston RL, Haque A, Bidani A. Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD). Chest. 1998 Oct;114(4 SUPPL.).
Lick, Scott D ; Alpard, S. K. ; Zwischenberger, J. B. ; Brunston, R. L. ; Haque, A. ; Bidani, A. / Conventional ventilation is superior to low- or high-frequency ventilation in the Non-Beating-Heart Lung Donor (NBHLD). In: Chest. 1998 ; Vol. 114, No. 4 SUPPL.
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AU - Haque, A.

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N2 - Purpose: The shortage of lung transplant donors may be lessened by the use of non-heart-beating lung donors (NBHLD). Optimal minute ventilation of the NBHLD is not known. We hypothesized that a moderately high minute ventilation relative to tissue metabolism to cause alveolar-induced respiratory alkalosis will ameliorate lung tissue acidosis and improve lung parenchyma viability. Methods: After sacrifice, Sprague-Dawley rats were separated into 4 groups of 8 rats each: deflated, non-ventilated controls (group I); 2 breaths/min (group II); 10 breaths/min (group III); and 20 breaths/min (group IV). All ventilation was done with room air and volumetrically set for full chest wall expansion. Two hours after sacrifice, the lungs were excised and flushed with tryphan blue vital dye to assess cell viability. Percent non-viable type II pneumocytes/10 HPF were counted in a blinded manner by a single pathologist. Results: GROUP % non-viable cells p value I (control) 37.6 ± 5.7 (I vs. II) p = 0.195 II (2 breath/min) 25.0 ± 3.7 (II vs. III) p = 0.002 III (10 breath/min) 7.7 ± 1.5 (I vs. III) p < 0.001 IV (20 breaths/min) 42.5 ± 4.3 (II vs. IV) p < 0.05 (III vs. IV) p < 0.001 Conclusions: Ventilation of the NBHLD at a moderately high rate (10 breath/min) relative to lung metabolism gives significantly improved cell viability over low frequency or high-frequency ventilation.

AB - Purpose: The shortage of lung transplant donors may be lessened by the use of non-heart-beating lung donors (NBHLD). Optimal minute ventilation of the NBHLD is not known. We hypothesized that a moderately high minute ventilation relative to tissue metabolism to cause alveolar-induced respiratory alkalosis will ameliorate lung tissue acidosis and improve lung parenchyma viability. Methods: After sacrifice, Sprague-Dawley rats were separated into 4 groups of 8 rats each: deflated, non-ventilated controls (group I); 2 breaths/min (group II); 10 breaths/min (group III); and 20 breaths/min (group IV). All ventilation was done with room air and volumetrically set for full chest wall expansion. Two hours after sacrifice, the lungs were excised and flushed with tryphan blue vital dye to assess cell viability. Percent non-viable type II pneumocytes/10 HPF were counted in a blinded manner by a single pathologist. Results: GROUP % non-viable cells p value I (control) 37.6 ± 5.7 (I vs. II) p = 0.195 II (2 breath/min) 25.0 ± 3.7 (II vs. III) p = 0.002 III (10 breath/min) 7.7 ± 1.5 (I vs. III) p < 0.001 IV (20 breaths/min) 42.5 ± 4.3 (II vs. IV) p < 0.05 (III vs. IV) p < 0.001 Conclusions: Ventilation of the NBHLD at a moderately high rate (10 breath/min) relative to lung metabolism gives significantly improved cell viability over low frequency or high-frequency ventilation.

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