Abstract
Cultured myotubes form clusters of acetylcholine receptors (AChRs) spontaneously and at sites of nerve-muscle contact. To investigate the cellular mechanisms by which spontaneous clusters are formed, we have made hybrid myotubes between a mouse muscle cell variant, S27, that does not cluster AChRs, and one that does not make AChRs. We have also made hybrid myotubes using S27 and quail muscle cells. In both cases, clusters of AChRs were found near the non-S27 nuclei; in the case of the interspecific hybrids, mouse AChRs were associated with extracellular matrix components contributed by the quail nuclei. Our results suggest that AChRs made by one nucleus can be clustered by localized extracellular matrix produced by a different nucleus and provide an example of nuclear cooperation between the products of different nuclei within multinucleated muscle fibers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6595-6598 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 89 |
| Issue number | 14 |
| State | Published - Jul 15 1992 |
Fingerprint
ASJC Scopus subject areas
- Genetics
- General
Cite this
Cooperation between the products of different nuclei in hybrid myotubes produces localized acetylcholine receptor clusters. / Gordon, Herman; Ralston, Evelyn; Hall, Zach W.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 14, 15.07.1992, p. 6595-6598.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cooperation between the products of different nuclei in hybrid myotubes produces localized acetylcholine receptor clusters
AU - Gordon, Herman
AU - Ralston, Evelyn
AU - Hall, Zach W.
PY - 1992/7/15
Y1 - 1992/7/15
N2 - Cultured myotubes form clusters of acetylcholine receptors (AChRs) spontaneously and at sites of nerve-muscle contact. To investigate the cellular mechanisms by which spontaneous clusters are formed, we have made hybrid myotubes between a mouse muscle cell variant, S27, that does not cluster AChRs, and one that does not make AChRs. We have also made hybrid myotubes using S27 and quail muscle cells. In both cases, clusters of AChRs were found near the non-S27 nuclei; in the case of the interspecific hybrids, mouse AChRs were associated with extracellular matrix components contributed by the quail nuclei. Our results suggest that AChRs made by one nucleus can be clustered by localized extracellular matrix produced by a different nucleus and provide an example of nuclear cooperation between the products of different nuclei within multinucleated muscle fibers.
AB - Cultured myotubes form clusters of acetylcholine receptors (AChRs) spontaneously and at sites of nerve-muscle contact. To investigate the cellular mechanisms by which spontaneous clusters are formed, we have made hybrid myotubes between a mouse muscle cell variant, S27, that does not cluster AChRs, and one that does not make AChRs. We have also made hybrid myotubes using S27 and quail muscle cells. In both cases, clusters of AChRs were found near the non-S27 nuclei; in the case of the interspecific hybrids, mouse AChRs were associated with extracellular matrix components contributed by the quail nuclei. Our results suggest that AChRs made by one nucleus can be clustered by localized extracellular matrix produced by a different nucleus and provide an example of nuclear cooperation between the products of different nuclei within multinucleated muscle fibers.
UR - http://www.scopus.com/inward/record.url?scp=0026696857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026696857&partnerID=8YFLogxK
M3 - Article
VL - 89
SP - 6595
EP - 6598
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 14
ER -