Cooperative bending of the 21-base-pair repeats of the SV40 viral early promoter by human Sp1

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The overall structural features of the multimeric complex between Sp1 and the 21-base-pair repeat of the early promoter region of SV40 DNA have been determined using hydroxyl-radical footprinting; (+)-CC-1065, a sequence-specific minor groove bending probe; and circularization experiments. The results show that the 21-base-pair repeat region has an intrinsically in-phase bent structure that is stabilized upon saturation Sp1 binding by protein-DNA and protein-protein interactions to produce a looping structure. The direction of the Sp1-stabilized bending of DNA occurs into the minor groove and is localized between each of the Sp1 binding sites. These results are used as the basis to propose a looping structure for the multimeric Sp1 21-base-pair repeat region of SV40 DNA. Last, these results provide a rationale for the recently observed inhibition of basal transcriptional levels by site-specific triple-helical DNA complexes.

Original languageEnglish (US)
Pages (from-to)9578-9587
Number of pages10
JournalBiochemistry
Volume33
Issue number32
StatePublished - 1994
Externally publishedYes

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Base Pairing
DNA
CC 1065
DNA-Binding Proteins
Genetic Promoter Regions
Hydroxyl Radical
Proteins
Binding Sites
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cooperative bending of the 21-base-pair repeats of the SV40 viral early promoter by human Sp1. / Sun, Daekyu; Hurley, Laurence.

In: Biochemistry, Vol. 33, No. 32, 1994, p. 9578-9587.

Research output: Contribution to journalArticle

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