Cooperative phenomena in two-pulse, two-color laser photocoagulation of cutaneous blood vessels

Jennifer K Barton, George Frangineas, Herbert Pummer, John F. Black

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

A novel laser system has been developed to study the effects of multiple laser pulses of differing wavelengths on cutaneous blood vessels in vivo, using the hamster dorsal skin flap preparation and in vitro, using cuvettes of whole or diluted blood. The system permits sequenced irradiation with well-defined intrapulse spacing at 532 nm, using a long-pulse frequency-doubled Nd:YAG laser, and at 1064 nm, using a long-pulse Nd:YAG laser. Using this system, we have identified a parameter space where two pulses of different wavelengths act in a synergistic manner to effect permanent vessel damage at radiant exposures where the two pulses individually have little or no effect. Using a two-color pump-probe technique in vitro, we have identified a phenomenon we call green-light-induced infrared absorption, where a pulse of green light causes photochemical and photothermal modifications to the chemical constituents of blood and results in enhanced infrared absorption. We identify a new chemical species, met-hemoglobin, not normally present in healthy human blood but formed during laser photocoagulation which we believe is implicated in the enhanced near-infrared absorption.

Original languageEnglish (US)
Pages (from-to)642-650
Number of pages9
JournalPhotochemistry and Photobiology
Volume73
Issue number6
DOIs
StatePublished - Jun 1 2001

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Light Coagulation
blood vessels
Blood vessels
Blood Vessels
Methyl Green
Laser pulses
Infrared absorption
Lasers
Color
Solid-State Lasers
color
Skin
Blood
pulses
infrared absorption
blood
lasers
Methemoglobin
Cricetinae
Wavelength

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Cooperative phenomena in two-pulse, two-color laser photocoagulation of cutaneous blood vessels. / Barton, Jennifer K; Frangineas, George; Pummer, Herbert; Black, John F.

In: Photochemistry and Photobiology, Vol. 73, No. 6, 01.06.2001, p. 642-650.

Research output: Contribution to journalArticle

Barton, Jennifer K ; Frangineas, George ; Pummer, Herbert ; Black, John F. / Cooperative phenomena in two-pulse, two-color laser photocoagulation of cutaneous blood vessels. In: Photochemistry and Photobiology. 2001 ; Vol. 73, No. 6. pp. 642-650.
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