COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain

Xing Wang Deng, Minami Matsui, Ning Wei, Doris Wagner, Angela M. Chu, Kenneth A Feldmann, Peter H. Quail

Research output: Contribution to journalArticle

392 Citations (Scopus)

Abstract

Plant seedling development is capable of following 1 of 2 distinct morphogenic pathways: skotomorphogenesis in darkness and photomorphogenesis in light. Dark-grown Arabidopsis seedlings with recessive mutations at the constitutively photomorphogenic (COP1) locus indicate that the wild-type COP1 protein represses photomorphogenesis in darkness and that light reverses this repressive activity. Using a T-DNA-tagged mutant, we have cloned the COP1 locus. The amino-terminal half of the encoded protein contains a conserved zinc-binding motif, whereas the carboxylterminal half contains a domain homologous to the WD-40 repeat motif of Gβ proteins. The presence of both a putative DNA-binding motif and a G protein-related domain in a single polypeptide suggests that COP1 may be the first of a new class of regulatory molecules. This novel structure could endow COP1 with the capacity to function as a negative transcriptional regulator capable of direct interaction with components of the G protein signaling pathway.

Original languageEnglish (US)
Pages (from-to)791-801
Number of pages11
JournalCell
Volume71
Issue number5
DOIs
StatePublished - Nov 27 1992

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Regulator Genes
GTP-Binding Proteins
Arabidopsis
Zinc
Genes
Darkness
Seedlings
Light
Nucleotide Motifs
Proteins
Plant Development
Peptides
Mutation
Molecules
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain. / Deng, Xing Wang; Matsui, Minami; Wei, Ning; Wagner, Doris; Chu, Angela M.; Feldmann, Kenneth A; Quail, Peter H.

In: Cell, Vol. 71, No. 5, 27.11.1992, p. 791-801.

Research output: Contribution to journalArticle

Deng, Xing Wang ; Matsui, Minami ; Wei, Ning ; Wagner, Doris ; Chu, Angela M. ; Feldmann, Kenneth A ; Quail, Peter H. / COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain. In: Cell. 1992 ; Vol. 71, No. 5. pp. 791-801.
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