Copper intoxication inhibits aerobic nucleotide synthesis in Streptococcus pneumoniae

Michael D.L. Johnson, Thomas E. Kehl-Fie, Jason W. Rosch

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Copper is universally toxic in excess, a feature exploited by the human immune system to facilitate bacterial clearance. The mechanism of copper intoxication remains unknown for many bacterial species. Here, we demonstrate that copper toxicity in Streptococcus pneumoniae is independent from oxidative stress but, rather, is the result of copper inhibiting the aerobic dNTP biosynthetic pathway. Furthermore, we show that copper-intoxicated S. pneumoniae is rescued by manganese, which is an essential metal in the aerobic nucleotide synthesis pathway. These data provide insight into new targets to enhance copper-mediated toxicity during bacterial clearance.

Original languageEnglish (US)
Pages (from-to)786-794
Number of pages9
JournalMetallomics
Volume7
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biophysics
  • Biomaterials
  • Biochemistry
  • Metals and Alloys

Fingerprint Dive into the research topics of 'Copper intoxication inhibits aerobic nucleotide synthesis in Streptococcus pneumoniae'. Together they form a unique fingerprint.

  • Cite this