Correlation between in vivo and in vitro pulmonary responses to jet propulsion fuel-8 using precision-cut lung slices and a dynamic organ culture system

Allison M. Hays, Robert Clark Lantz, Mark L. Witten

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In tissue slice models, interactions between the heterogeneous cell types comprising the lung parenchyma are maintained thus providing a controlled system for the study of pulmonary toxicology in vitro. However, validation of the model in vitro system must be affirmed. Previous reports, in in vivo systems, have demonstrated that Clara cells and alveolar type II cells are the targets following inhalation of JP-8 jet fuel. We have utilized the lung slice model to determine if cellular targets are similar following in vitro exposure to JP-8. Agar-filled adult rat lung explants were cored and precision cut, using the Brendel/Vitron tissue slicer. Slices were cultured on titanium screens located as half-cylinders in cylindrical Teflon cradles that were loaded into standard scintillation vials and incubated at 37°C. Slices were exposed to JP-8 jet fuel (0.5 mg/ml, 1.0 mg/ml, and 1.5 mg/ml in medium) for up to 24 hours. We determined ATP content using a luciferin-luciferase bioluminescent assay. No significant difference was found between the JP-8 jet fuel doses or time points, when compared to controls. Results were correlated with structural alterations following aerosol inhalation of JP-8. As a general observation, ultrastructural evaluation of alveolar type II cells revealed an apparent increase in the number and size of surfactant secreting lamellar bodies that was JP-8 jet fuel-dose dependent. These results are similar to those observed following aerosol inhalation exposure. Thus, the lung tissue slice model appears to mimic in vivo effects of JP-8 and therefore is a useful model system for studying the mechanisms of lung injury following JP-8 exposure.

Original languageEnglish (US)
Pages (from-to)200-207
Number of pages8
JournalToxicologic Pathology
Volume31
Issue number2
DOIs
StatePublished - Mar 2003

Fingerprint

Organ Culture Techniques
Propulsion
Lung
Alveolar Epithelial Cells
Tissue
Aerosols
Inhalation
Luminescent Measurements
Inhalation Exposure
Polytetrafluoroethylene
Lung Injury
Scintillation
Titanium
Luciferases
Surface-Active Agents
Toxicology
Agar
Rats
Assays
Adenosine Triphosphate

Keywords

  • Aerosol
  • Clara cell
  • Glutathione
  • Kerosene
  • Type II cell

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Correlation between in vivo and in vitro pulmonary responses to jet propulsion fuel-8 using precision-cut lung slices and a dynamic organ culture system. / Hays, Allison M.; Lantz, Robert Clark; Witten, Mark L.

In: Toxicologic Pathology, Vol. 31, No. 2, 03.2003, p. 200-207.

Research output: Contribution to journalArticle

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